MDM2 antagonists promote CRISPR/Cas9-mediated precise genome editing in sheep primary cells

MDM2 antagonists promote CRISPR/Cas9-mediated precise genome editing in sheep primary cells

CRISPR-Cas9-mediated genome editing in sheep is of great use in both agricultural and biomedical applications. While targeted gene knockout by CRISPR-Cas9 through non-homologous end joining (NHEJ) has worked efficiently, the knockin efficiency via homology-directed repair (HDR) remains lower, which...

Full description

Bibliographic Details
Main Authors: Yan Li, Di Lian, Jiahao Wang, Yue Zhao, Yao Li, Guoshi Liu, Sen Wu, Shoulong Deng, Xuguang Du, Zhengxing Lian
Format: Article
Language:English
Published: Elsevier 2023-03-01
Series:Molecular Therapy: Nucleic Acids
Subjects:
MT: RNA/DNA Editing
CRISPR-Cas9
homology-directed repair
p53
MDM2 antagonists
cell cycle
Online Access:http://www.sciencedirect.com/science/article/pii/S2162253123000033

Internet

http://www.sciencedirect.com/science/article/pii/S2162253123000033

Similar Items