PCV2 Triggers PK-15 Cell Apoptosis Through the PLC–IP3R–Ca2+ Signaling Pathway
The endoplasmic reticulum (ER) plays an essential role in Ca2+ concentration balance and protein biosynthesis. During infection, the virus needs to complete its life process with the help of ER. At the same time, ER also produces ER stress (ERS), which induces apoptosis to resist virus infection. Ou...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-06-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.674907/full |
| _version_ | 1818436714898653184 |
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| author | Shuo Wang Shuo Wang Chen Li Panpan Sun Jianli Shi Xiaoyan Wu Xiaoyan Wu Chang Liu Zhe Peng Hong Han Shaojian Xu Ying Yang Ying Yang Yao Tian Yao Tian Jiaxin Li Jiaxin Li Hongbin He Jun Li Jun Li Jun Li Zhao Wang |
| author_facet | Shuo Wang Shuo Wang Chen Li Panpan Sun Jianli Shi Xiaoyan Wu Xiaoyan Wu Chang Liu Zhe Peng Hong Han Shaojian Xu Ying Yang Ying Yang Yao Tian Yao Tian Jiaxin Li Jiaxin Li Hongbin He Jun Li Jun Li Jun Li Zhao Wang |
| author_sort | Shuo Wang |
| collection | DOAJ |
| description | The endoplasmic reticulum (ER) plays an essential role in Ca2+ concentration balance and protein biosynthesis. During infection, the virus needs to complete its life process with the help of ER. At the same time, ER also produces ER stress (ERS), which induces apoptosis to resist virus infection. Our study explored the Ca2+ concentration, ERS, and the apoptosis mechanism after porcine circovirus 2 (PCV2) infection. We show here that PCV2 infection induces the increased cytoplasmic Ca2+ level and PK-15 cell ER swelling. The colocalization of phospholipase C (PLC) and inositol 1,4,5-trisphosphate receptor (IP3R) in the cytoplasm was observed by laser confocal microscopy. Western blot and quantitative polymerase chain reaction experiments confirmed that PLC and IP3R expression levels increased after PCV2 infection, and Ca2+ concentration in the cytoplasm increased after virus infection. These results suggest that PCV2 infection triggers ERS of PK-15 cells via the PLC–IP3R–Ca2+ signaling pathway to promote the release of intracellular Ca2+ and led to cell apoptosis. |
| first_indexed | 2024-12-14T17:13:10Z |
| format | Article |
| id | doaj.art-8f71ae8e986b4d378bfd3ca8c16f5cdd |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-14T17:13:10Z |
| publishDate | 2021-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-8f71ae8e986b4d378bfd3ca8c16f5cdd2022-12-21T22:53:29ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-06-011210.3389/fmicb.2021.674907674907PCV2 Triggers PK-15 Cell Apoptosis Through the PLC–IP3R–Ca2+ Signaling PathwayShuo Wang0Shuo Wang1Chen Li2Panpan Sun3Jianli Shi4Xiaoyan Wu5Xiaoyan Wu6Chang Liu7Zhe Peng8Hong Han9Shaojian Xu10Ying Yang11Ying Yang12Yao Tian13Yao Tian14Jiaxin Li15Jiaxin Li16Hongbin He17Jun Li18Jun Li19Jun Li20Zhao Wang21Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, ChinaCollege of Life Sciences, Shandong Normal University, Jinan, ChinaShandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, ChinaQingdao Agricultural University, Qingdao, ChinaShandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, ChinaShandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, ChinaCollege of Life Sciences, Shandong Normal University, Jinan, ChinaShandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, ChinaShandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, ChinaShandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, ChinaShandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, ChinaShandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, ChinaCollege of Life Sciences, Shandong Normal University, Jinan, ChinaShandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, ChinaQingdao Agricultural University, Qingdao, ChinaShandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, ChinaQingdao Agricultural University, Qingdao, ChinaCollege of Life Sciences, Shandong Normal University, Jinan, ChinaShandong Provincial Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine Shandong Academy of Agricultural Sciences, Jinan, ChinaCollege of Life Sciences, Shandong Normal University, Jinan, ChinaQingdao Agricultural University, Qingdao, ChinaChina Institute of Veterinary Drug Control, Beijing, ChinaThe endoplasmic reticulum (ER) plays an essential role in Ca2+ concentration balance and protein biosynthesis. During infection, the virus needs to complete its life process with the help of ER. At the same time, ER also produces ER stress (ERS), which induces apoptosis to resist virus infection. Our study explored the Ca2+ concentration, ERS, and the apoptosis mechanism after porcine circovirus 2 (PCV2) infection. We show here that PCV2 infection induces the increased cytoplasmic Ca2+ level and PK-15 cell ER swelling. The colocalization of phospholipase C (PLC) and inositol 1,4,5-trisphosphate receptor (IP3R) in the cytoplasm was observed by laser confocal microscopy. Western blot and quantitative polymerase chain reaction experiments confirmed that PLC and IP3R expression levels increased after PCV2 infection, and Ca2+ concentration in the cytoplasm increased after virus infection. These results suggest that PCV2 infection triggers ERS of PK-15 cells via the PLC–IP3R–Ca2+ signaling pathway to promote the release of intracellular Ca2+ and led to cell apoptosis.https://www.frontiersin.org/articles/10.3389/fmicb.2021.674907/fullapoptosisporcine circovirus 2PLCIP3RCacpsdummy2+endoplasmic reticulum stress |
| spellingShingle | Shuo Wang Shuo Wang Chen Li Panpan Sun Jianli Shi Xiaoyan Wu Xiaoyan Wu Chang Liu Zhe Peng Hong Han Shaojian Xu Ying Yang Ying Yang Yao Tian Yao Tian Jiaxin Li Jiaxin Li Hongbin He Jun Li Jun Li Jun Li Zhao Wang PCV2 Triggers PK-15 Cell Apoptosis Through the PLC–IP3R–Ca2+ Signaling Pathway Frontiers in Microbiology apoptosis porcine circovirus 2 PLC IP3R Cacpsdummy2+ endoplasmic reticulum stress |
| title | PCV2 Triggers PK-15 Cell Apoptosis Through the PLC–IP3R–Ca2+ Signaling Pathway |
| title_full | PCV2 Triggers PK-15 Cell Apoptosis Through the PLC–IP3R–Ca2+ Signaling Pathway |
| title_fullStr | PCV2 Triggers PK-15 Cell Apoptosis Through the PLC–IP3R–Ca2+ Signaling Pathway |
| title_full_unstemmed | PCV2 Triggers PK-15 Cell Apoptosis Through the PLC–IP3R–Ca2+ Signaling Pathway |
| title_short | PCV2 Triggers PK-15 Cell Apoptosis Through the PLC–IP3R–Ca2+ Signaling Pathway |
| title_sort | pcv2 triggers pk 15 cell apoptosis through the plc ip3r ca2 signaling pathway |
| topic | apoptosis porcine circovirus 2 PLC IP3R Cacpsdummy2+ endoplasmic reticulum stress |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.674907/full |
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