Profiling the antibody response of humans protected by immunization with Plasmodium vivax radiation-attenuated sporozoites
Abstract Malaria sterile immunity has been reproducibly induced by immunization with Plasmodium radiation-attenuated sporozoites (RAS). Analyses of sera from RAS-immunized individuals allowed the identification of P. falciparum antigens, such as the circumsporozoite protein (CSP), the basis for the...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-53175-0 |
| _version_ | 1797275219270828032 |
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| author | Mary Lopez-Perez Aarti Jain D. Huw Davies Juan M. Vásquez-Jiménez Sonia M. Herrera José Oñate Philip L. Felgner Sócrates Herrera Myriam Arévalo-Herrera |
| author_facet | Mary Lopez-Perez Aarti Jain D. Huw Davies Juan M. Vásquez-Jiménez Sonia M. Herrera José Oñate Philip L. Felgner Sócrates Herrera Myriam Arévalo-Herrera |
| author_sort | Mary Lopez-Perez |
| collection | DOAJ |
| description | Abstract Malaria sterile immunity has been reproducibly induced by immunization with Plasmodium radiation-attenuated sporozoites (RAS). Analyses of sera from RAS-immunized individuals allowed the identification of P. falciparum antigens, such as the circumsporozoite protein (CSP), the basis for the RTS, S and R21Matrix-M vaccines. Similar advances in P. vivax (Pv) vaccination have been elusive. We previously reported 42% (5/12) of sterile protection in malaria-unexposed, Duffy-positive (Fy +) volunteers immunized with PvRAS followed by a controlled human malaria infection (CHMI). Using a custom protein microarray displaying 515 Pv antigens, we found a significantly higher reactivity to PvCSP and one hypothetical protein (PVX_089630) in volunteers protected against P. vivax infection. In mock-vaccinated Fy + volunteers, a strong antibody response to CHMI was also observed. Although the Fy- volunteers immunized with non-irradiated Pv-infected mosquitoes (live sporozoites) did not develop malaria after CHMI, they recognized a high number of antigens, indicating the temporary presence of asexual parasites in peripheral blood. Together, our findings contribute to the understanding of the antibody response to P. vivax infection and allow the identification of novel parasite antigens as vaccine candidates. Trial registration: ClinicalTrials.gov number: NCT 01082341. |
| first_indexed | 2024-03-07T15:10:15Z |
| format | Article |
| id | doaj.art-8f73505508e7404eae3cdc664a68ee12 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-07T15:10:15Z |
| publishDate | 2024-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-8f73505508e7404eae3cdc664a68ee122024-03-05T18:40:22ZengNature PortfolioScientific Reports2045-23222024-02-0114111210.1038/s41598-024-53175-0Profiling the antibody response of humans protected by immunization with Plasmodium vivax radiation-attenuated sporozoitesMary Lopez-Perez0Aarti Jain1D. Huw Davies2Juan M. Vásquez-Jiménez3Sonia M. Herrera4José Oñate5Philip L. Felgner6Sócrates Herrera7Myriam Arévalo-Herrera8Malaria Vaccine and Drug Development Center (MVDC)Department Physiology & Biophysics, Vaccine R&D Center, University of California IrvineDepartment Physiology & Biophysics, Vaccine R&D Center, University of California IrvineMalaria Vaccine and Drug Development Center (MVDC)Caucaseco Scientific Research CenterImbanaco - QuironSaludDepartment Physiology & Biophysics, Vaccine R&D Center, University of California IrvineMalaria Vaccine and Drug Development Center (MVDC)Malaria Vaccine and Drug Development Center (MVDC)Abstract Malaria sterile immunity has been reproducibly induced by immunization with Plasmodium radiation-attenuated sporozoites (RAS). Analyses of sera from RAS-immunized individuals allowed the identification of P. falciparum antigens, such as the circumsporozoite protein (CSP), the basis for the RTS, S and R21Matrix-M vaccines. Similar advances in P. vivax (Pv) vaccination have been elusive. We previously reported 42% (5/12) of sterile protection in malaria-unexposed, Duffy-positive (Fy +) volunteers immunized with PvRAS followed by a controlled human malaria infection (CHMI). Using a custom protein microarray displaying 515 Pv antigens, we found a significantly higher reactivity to PvCSP and one hypothetical protein (PVX_089630) in volunteers protected against P. vivax infection. In mock-vaccinated Fy + volunteers, a strong antibody response to CHMI was also observed. Although the Fy- volunteers immunized with non-irradiated Pv-infected mosquitoes (live sporozoites) did not develop malaria after CHMI, they recognized a high number of antigens, indicating the temporary presence of asexual parasites in peripheral blood. Together, our findings contribute to the understanding of the antibody response to P. vivax infection and allow the identification of novel parasite antigens as vaccine candidates. Trial registration: ClinicalTrials.gov number: NCT 01082341.https://doi.org/10.1038/s41598-024-53175-0 |
| spellingShingle | Mary Lopez-Perez Aarti Jain D. Huw Davies Juan M. Vásquez-Jiménez Sonia M. Herrera José Oñate Philip L. Felgner Sócrates Herrera Myriam Arévalo-Herrera Profiling the antibody response of humans protected by immunization with Plasmodium vivax radiation-attenuated sporozoites Scientific Reports |
| title | Profiling the antibody response of humans protected by immunization with Plasmodium vivax radiation-attenuated sporozoites |
| title_full | Profiling the antibody response of humans protected by immunization with Plasmodium vivax radiation-attenuated sporozoites |
| title_fullStr | Profiling the antibody response of humans protected by immunization with Plasmodium vivax radiation-attenuated sporozoites |
| title_full_unstemmed | Profiling the antibody response of humans protected by immunization with Plasmodium vivax radiation-attenuated sporozoites |
| title_short | Profiling the antibody response of humans protected by immunization with Plasmodium vivax radiation-attenuated sporozoites |
| title_sort | profiling the antibody response of humans protected by immunization with plasmodium vivax radiation attenuated sporozoites |
| url | https://doi.org/10.1038/s41598-024-53175-0 |
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