Simultaneous Improvement in the Thermostability and Catalytic Activity of Epoxidase Lsd18 for the Synthesis of Lasalocid A

Simultaneous Improvement in the Thermostability and Catalytic Activity of Epoxidase Lsd18 for the Synthesis of Lasalocid A

Enzymes used in the synthesis of natural products are potent catalysts, capable of efficient and stereoselective chemical transformations. Lsd18 catalyzes two sequential epoxidations during the biosynthesis of lasalocid A, a polyether polyketide natural product. We performed protein engineering on L...

Full description

Bibliographic Details
Main Authors: Ning Liu, Hongli Xiao, Yongjian Zang, Longji Zhou, Jun Mencius, Zhiwei Yang, Shu Quan, Xi Chen
Format: Article
Language:English
Published: MDPI AG 2023-11-01
Series:International Journal of Molecular Sciences
Subjects:
natural product
Lsd18
thermostability
FoldX
Rossetta-ddG
catalytic activity
Online Access:https://www.mdpi.com/1422-0067/24/23/16795

Internet

https://www.mdpi.com/1422-0067/24/23/16795

Similar Items