A comparison between enzyme immunoassay and HPLC for ochratoxin A detection in green, roasted and instant coffee

A comparison between enzyme immunoassay and HPLC for ochratoxin A detection in green, roasted and instant coffee

An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for ochratoxin A (OTA) detection in green, roasted and instant coffees was developed using anti-OTA monoclonal antibody. Immunological reagents prepared were OTA-BSA (4.76 mg/mL), anti-OTA.7 MAb (2x10³-fold dilution) and HRP-anti I...

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Bibliographic Details
Main Authors: Simone Fujii, Elisabete Yurie Sataque Ono, Ricardo Marcelo Reche Ribeiro, Fernanda Garcia Algarte Assunção, Cássia Reika Takabayashi, Tereza Cristina Rocha Moreira de Oliveira, Eiko Nakagawa Itano, Yoshio Ueno, Osamu Kawamura, Elisa Yoko Hirooka
Format: Article
Language:English
Published: Instituto de Tecnologia do Paraná (Tecpar) 2007-03-01
Series:Brazilian Archives of Biology and Technology
Subjects:
Ochratoxin
immunoassay
HPLC
monoclonal antibody
coffee
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200020

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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200020

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