Protein and antibody purification followed by immunoprecipitation of MYB and GATA zinc finger-type maize proteins with magnetic beads

Protein and antibody purification followed by immunoprecipitation of MYB and GATA zinc finger-type maize proteins with magnetic beads

Summary: Co-immunoprecipitation (Co-IP) is a widely used and powerful approach for studying protein-protein interactions in vivo. Here, we describe a protocol for antibody purification and immobilization followed by immunoprecipitation from plant tissue extracts using magnetic beads. The protocol ha...

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Bibliographic Details
Main Authors: Isabel Cristina Vélez-Bermúdez, Jorge Enrique Salazar-Henao, Marta Riera, David Caparros-Ruiz, Wolfgang Schmidt
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:STAR Protocols
Subjects:
Plant sciences
Molecular Biology
Protein Biochemistry
Protein expression and purification
Online Access:http://www.sciencedirect.com/science/article/pii/S266616672200329X

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http://www.sciencedirect.com/science/article/pii/S266616672200329X

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