High-throughput sequencing SELEX for the determination of DNA-binding protein specificities in vitro

High-throughput sequencing SELEX for the determination of DNA-binding protein specificities in vitro

Summary: High-throughput sequencing SELEX (HT-SELEX) is a powerful technique for unbiased determination of preferred target motifs of DNA-binding proteins in vitro. The procedure depends upon selection of DNA binding sites from a random library of oligonucleotides by purifying protein-DNA complexes...

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Bibliographic Details
Main Authors: Raphaël Pantier, Kashyap Chhatbar, Grace Alston, Heng Yang Lee, Adrian Bird
Format: Article
Language:English
Published: Elsevier 2022-09-01
Series:STAR Protocols
Subjects:
Bioinformatics
Sequence analysis
Sequencing
High Throughput Screening
Molecular Biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722003707

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http://www.sciencedirect.com/science/article/pii/S2666166722003707

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