Optimization of Cas9 RNA sequence to reduce its unexpected effects as a microRNA sponge

Optimization of Cas9 RNA sequence to reduce its unexpected effects as a microRNA sponge

Highlights Cas9 RNA functions as a miRNA sponge. Let-7 is the dominant regulated miRNA by Cas9 RNA. RNA sequence optimization of Cas9 by synonymous mutation improves its safety.

Bibliographic Details
Main Authors: Junfeng Jiang, Tao Zeng, Li Zhang, Xingfei Fan, Qishu Jin, Haitao Ni, Yusheng Ye, Lipeng Cheng, Li Li, Liujun Wang, Sha Xu, Yu Yang, Juan Gu, Bing Guo, Lei Wang, Xin Li, Yingyi Qin, Jiaxi Li, Jinjiang Wang, Xi Chen, Minjuan Wu, Qi-long Ying, Xingjun Qin, Yefei Wang, Yue Wang
Format: Article
Language:English
Published: BMC 2022-06-01
Series:Molecular Cancer
Subjects:
CRISPR–Cas9
miRNA sponge
Let-7
RNA sequence optimization
Online Access:https://doi.org/10.1186/s12943-022-01604-x

Internet

https://doi.org/10.1186/s12943-022-01604-x

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