Protocol for density gradient neutrophil isolation and flow cytometry-based characterization from human peripheral blood

Summary: Neutrophils are the first immune responders to bacterial or viral infection and play key roles in the host immune response; however, handling and investigating fresh neutrophils can be challenging. Here, we present a protocol for isolating neutrophils from the peripheral blood of healthy do...

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Bibliographic Details
Main Authors: Yi Kuang, Upasana Parthasarathy, Roberta Martinelli
Format: Article
Language:English
Published: Elsevier 2023-09-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723004641