Protocols to culture and harvest hepatic tumor organoids for metabolic assays

Summary: Three-dimensional organoids, which resemble the pathophysiology and structural architecture of the original tissues, are a preferable in vitro model system for assessing metabolic activities in response to various environmental or nutritional changes. Here, we describe step-by-step protocol...

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Bibliographic Details
Main Authors: Man Tong, Stephanie Ma
Format: Article
Language:English
Published: Elsevier 2022-09-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722004774