WDPCP Modulates Cilia Beating Through the MAPK/ERK Pathway in Chronic Rhinosinusitis With Nasal Polyps
Cilia loss and dysfunction is one of the typical pathological features of chronic rhinosinusitis with nasal polyps (CRSwNP). Tryptophan-aspartic acid (W-D) repeat containing planar cell polarity effector (WDPCP) has been proven to be an essential element for ciliogenesis in human nasal epithelium, b...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-02-01
|
| Series: | Frontiers in Cell and Developmental Biology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2020.630340/full |
| _version_ | 1818655770310344704 |
|---|---|
| author | Yun Ma Peng Tian Hua Zhong Fan Wu Qining Zhang Xiang Liu Hua Dang Qiujian Chen Hua Zou Yiqing Zheng |
| author_facet | Yun Ma Peng Tian Hua Zhong Fan Wu Qining Zhang Xiang Liu Hua Dang Qiujian Chen Hua Zou Yiqing Zheng |
| author_sort | Yun Ma |
| collection | DOAJ |
| description | Cilia loss and dysfunction is one of the typical pathological features of chronic rhinosinusitis with nasal polyps (CRSwNP). Tryptophan-aspartic acid (W-D) repeat containing planar cell polarity effector (WDPCP) has been proven to be an essential element for ciliogenesis in human nasal epithelium, but its role in the beating of cilia remains unclear. In this study, we sought to investigate the role of WDPCP and its underlying mechanism behind the dysfunction in the beating of cilia in nasal polyp tissue. We demonstrated WDPCP expression in the epithelium of nasal polyps. We also investigated the MAPK/ERK pathway in primary human sinonasal epithelial cells to explore the function of WDPCP. The air–liquid interface culture system was used as a model to verify the role of WDPCP and the MAPK/ERK pathway in the beating of cilia. With the dysfunction of cilia beating, we observed a low expression of WDPCP in the epithelium of nasal polyp tissues. Within the in vitro study, we found that WDPCP was critical for mitochondrial biogenesis and mitochondrial function in human sinonasal epithelial cells, possibly due to the activation of the MAPK/ERK pathway. The mitochondrial dysfunction caused by U0126 or lacking WDPCP could be partially recovered by dexamethasone. The low expression of WDPCP in nasal epithelium could affect mitochondria via the MAPK/ERK pathway, which may contribute to the dysfunction in the beating of cilia in CRSwNP. |
| first_indexed | 2024-12-17T03:14:58Z |
| format | Article |
| id | doaj.art-9010a26a651746d0a23e3ddfb1d5ca1b |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-17T03:14:58Z |
| publishDate | 2021-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-9010a26a651746d0a23e3ddfb1d5ca1b2022-12-21T22:05:41ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2021-02-01810.3389/fcell.2020.630340630340WDPCP Modulates Cilia Beating Through the MAPK/ERK Pathway in Chronic Rhinosinusitis With Nasal PolypsYun Ma0Peng Tian1Hua Zhong2Fan Wu3Qining Zhang4Xiang Liu5Hua Dang6Qiujian Chen7Hua Zou8Yiqing Zheng9Department of Otorhinolaryngology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, ChinaDepartment of Otorhinolaryngology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, ChinaDepartment of Otorhinolaryngology, First Affiliated Hospital of Sun Yat-sen University, Sun Yat-sen University, Guangzhou, ChinaDepartment of Otorhinolaryngology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, ChinaDepartment of Otorhinolaryngology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, ChinaDepartment of Otorhinolaryngology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, ChinaDepartment of Otorhinolaryngology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, ChinaDepartment of Otorhinolaryngology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, ChinaDepartment of Otorhinolaryngology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, ChinaDepartment of Otorhinolaryngology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, ChinaCilia loss and dysfunction is one of the typical pathological features of chronic rhinosinusitis with nasal polyps (CRSwNP). Tryptophan-aspartic acid (W-D) repeat containing planar cell polarity effector (WDPCP) has been proven to be an essential element for ciliogenesis in human nasal epithelium, but its role in the beating of cilia remains unclear. In this study, we sought to investigate the role of WDPCP and its underlying mechanism behind the dysfunction in the beating of cilia in nasal polyp tissue. We demonstrated WDPCP expression in the epithelium of nasal polyps. We also investigated the MAPK/ERK pathway in primary human sinonasal epithelial cells to explore the function of WDPCP. The air–liquid interface culture system was used as a model to verify the role of WDPCP and the MAPK/ERK pathway in the beating of cilia. With the dysfunction of cilia beating, we observed a low expression of WDPCP in the epithelium of nasal polyp tissues. Within the in vitro study, we found that WDPCP was critical for mitochondrial biogenesis and mitochondrial function in human sinonasal epithelial cells, possibly due to the activation of the MAPK/ERK pathway. The mitochondrial dysfunction caused by U0126 or lacking WDPCP could be partially recovered by dexamethasone. The low expression of WDPCP in nasal epithelium could affect mitochondria via the MAPK/ERK pathway, which may contribute to the dysfunction in the beating of cilia in CRSwNP.https://www.frontiersin.org/articles/10.3389/fcell.2020.630340/fullWDPCPcilia beatingmitochondriaMAPK/ERK pathwaychronic rhinosinusitis with nasal polyps (CRSwNP) |
| spellingShingle | Yun Ma Peng Tian Hua Zhong Fan Wu Qining Zhang Xiang Liu Hua Dang Qiujian Chen Hua Zou Yiqing Zheng WDPCP Modulates Cilia Beating Through the MAPK/ERK Pathway in Chronic Rhinosinusitis With Nasal Polyps Frontiers in Cell and Developmental Biology WDPCP cilia beating mitochondria MAPK/ERK pathway chronic rhinosinusitis with nasal polyps (CRSwNP) |
| title | WDPCP Modulates Cilia Beating Through the MAPK/ERK Pathway in Chronic Rhinosinusitis With Nasal Polyps |
| title_full | WDPCP Modulates Cilia Beating Through the MAPK/ERK Pathway in Chronic Rhinosinusitis With Nasal Polyps |
| title_fullStr | WDPCP Modulates Cilia Beating Through the MAPK/ERK Pathway in Chronic Rhinosinusitis With Nasal Polyps |
| title_full_unstemmed | WDPCP Modulates Cilia Beating Through the MAPK/ERK Pathway in Chronic Rhinosinusitis With Nasal Polyps |
| title_short | WDPCP Modulates Cilia Beating Through the MAPK/ERK Pathway in Chronic Rhinosinusitis With Nasal Polyps |
| title_sort | wdpcp modulates cilia beating through the mapk erk pathway in chronic rhinosinusitis with nasal polyps |
| topic | WDPCP cilia beating mitochondria MAPK/ERK pathway chronic rhinosinusitis with nasal polyps (CRSwNP) |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2020.630340/full |
| work_keys_str_mv | AT yunma wdpcpmodulatesciliabeatingthroughthemapkerkpathwayinchronicrhinosinusitiswithnasalpolyps AT pengtian wdpcpmodulatesciliabeatingthroughthemapkerkpathwayinchronicrhinosinusitiswithnasalpolyps AT huazhong wdpcpmodulatesciliabeatingthroughthemapkerkpathwayinchronicrhinosinusitiswithnasalpolyps AT fanwu wdpcpmodulatesciliabeatingthroughthemapkerkpathwayinchronicrhinosinusitiswithnasalpolyps AT qiningzhang wdpcpmodulatesciliabeatingthroughthemapkerkpathwayinchronicrhinosinusitiswithnasalpolyps AT xiangliu wdpcpmodulatesciliabeatingthroughthemapkerkpathwayinchronicrhinosinusitiswithnasalpolyps AT huadang wdpcpmodulatesciliabeatingthroughthemapkerkpathwayinchronicrhinosinusitiswithnasalpolyps AT qiujianchen wdpcpmodulatesciliabeatingthroughthemapkerkpathwayinchronicrhinosinusitiswithnasalpolyps AT huazou wdpcpmodulatesciliabeatingthroughthemapkerkpathwayinchronicrhinosinusitiswithnasalpolyps AT yiqingzheng wdpcpmodulatesciliabeatingthroughthemapkerkpathwayinchronicrhinosinusitiswithnasalpolyps |