RSPO3 impairs barrier function of human vascular endothelial monolayers and synergizes with pro-inflammatory IL-1
Abstract Background Endothelial barrier dysfunction characterized by hyperpermeability of the vascular endothelium is a key factor in the pathogenesis of chronic inflammatory diseases and affects clinical outcomes. In states of chronic inflammation, mediators secreted by activated immune cells or va...
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| Format: | Article |
| Language: | English |
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BMC
2018-08-01
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| Series: | Molecular Medicine |
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| Online Access: | http://link.springer.com/article/10.1186/s10020-018-0048-z |
| _version_ | 1811198806254944256 |
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| author | Tom Skaria Esther Bachli Gabriele Schoedon |
| author_facet | Tom Skaria Esther Bachli Gabriele Schoedon |
| author_sort | Tom Skaria |
| collection | DOAJ |
| description | Abstract Background Endothelial barrier dysfunction characterized by hyperpermeability of the vascular endothelium is a key factor in the pathogenesis of chronic inflammatory diseases and affects clinical outcomes. In states of chronic inflammation, mediators secreted by activated immune cells or vascular endothelium may affect the barrier function and permeability of the vascular endothelium. The matricellular R-spondin family member RSPO3 is produced by inflammatory-activated human monocytes and vascular endothelial cells, but its effects in the regulation of vascular endothelial barrier function remains elusive. Methods The present study investigates the effects of RSPO3 on the barrier function of adult human primary macro- and micro- vascular endothelial monolayers. Tight monolayers of primary endothelial cells from human coronary and pulmonary arteries, and cardiac, brain, and dermal microvascular beds were treated with RSPO3 either alone or in combination with pro-inflammatory mediator IL-1β. Endothelial barrier function was assessed non-invasively in real-time using Electric Cell-substrate Impedance Sensing. Results RSPO3 treatment critically affected barrier function by enhancing the permeability of all vascular endothelial monolayers investigated. To confer hyperpermeable phenotype in vascular endothelial monolayers, RSPO3 induced inter-endothelial gap formation by disrupting the β-catenin and VE-cadherin alignment at adherens junctions. RSPO3 synergistically enhanced the barrier impairing properties of the pro-inflammatory mediator IL-1β. Conclusion Here, we show that the matricellular protein RSPO3 is a mediator of endothelial hyperpermeability that can act in synergy with the inflammatory mediator IL-1β. This finding stimulates further studies to delineate the endothelial barrier impairing properties of RSPO3 and its synergistic interaction with IL-1β in chronic inflammatory diseases. |
| first_indexed | 2024-04-12T01:37:26Z |
| format | Article |
| id | doaj.art-9021ccd64df44f2695728b2c3fbf89b5 |
| institution | Directory Open Access Journal |
| issn | 1076-1551 1528-3658 |
| language | English |
| last_indexed | 2024-04-12T01:37:26Z |
| publishDate | 2018-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Medicine |
| spelling | doaj.art-9021ccd64df44f2695728b2c3fbf89b52022-12-22T03:53:17ZengBMCMolecular Medicine1076-15511528-36582018-08-012411710.1186/s10020-018-0048-zRSPO3 impairs barrier function of human vascular endothelial monolayers and synergizes with pro-inflammatory IL-1Tom Skaria0Esther Bachli1Gabriele Schoedon2Inflammation Research Unit, Division of Internal Medicine, University Hospital ZürichDepartment of Medicine, Uster HospitalInflammation Research Unit, Division of Internal Medicine, University Hospital ZürichAbstract Background Endothelial barrier dysfunction characterized by hyperpermeability of the vascular endothelium is a key factor in the pathogenesis of chronic inflammatory diseases and affects clinical outcomes. In states of chronic inflammation, mediators secreted by activated immune cells or vascular endothelium may affect the barrier function and permeability of the vascular endothelium. The matricellular R-spondin family member RSPO3 is produced by inflammatory-activated human monocytes and vascular endothelial cells, but its effects in the regulation of vascular endothelial barrier function remains elusive. Methods The present study investigates the effects of RSPO3 on the barrier function of adult human primary macro- and micro- vascular endothelial monolayers. Tight monolayers of primary endothelial cells from human coronary and pulmonary arteries, and cardiac, brain, and dermal microvascular beds were treated with RSPO3 either alone or in combination with pro-inflammatory mediator IL-1β. Endothelial barrier function was assessed non-invasively in real-time using Electric Cell-substrate Impedance Sensing. Results RSPO3 treatment critically affected barrier function by enhancing the permeability of all vascular endothelial monolayers investigated. To confer hyperpermeable phenotype in vascular endothelial monolayers, RSPO3 induced inter-endothelial gap formation by disrupting the β-catenin and VE-cadherin alignment at adherens junctions. RSPO3 synergistically enhanced the barrier impairing properties of the pro-inflammatory mediator IL-1β. Conclusion Here, we show that the matricellular protein RSPO3 is a mediator of endothelial hyperpermeability that can act in synergy with the inflammatory mediator IL-1β. This finding stimulates further studies to delineate the endothelial barrier impairing properties of RSPO3 and its synergistic interaction with IL-1β in chronic inflammatory diseases.http://link.springer.com/article/10.1186/s10020-018-0048-zEndothelial dysfunctionRSPO3InflammationVascular leakageIL-1β |
| spellingShingle | Tom Skaria Esther Bachli Gabriele Schoedon RSPO3 impairs barrier function of human vascular endothelial monolayers and synergizes with pro-inflammatory IL-1 Molecular Medicine Endothelial dysfunction RSPO3 Inflammation Vascular leakage IL-1β |
| title | RSPO3 impairs barrier function of human vascular endothelial monolayers and synergizes with pro-inflammatory IL-1 |
| title_full | RSPO3 impairs barrier function of human vascular endothelial monolayers and synergizes with pro-inflammatory IL-1 |
| title_fullStr | RSPO3 impairs barrier function of human vascular endothelial monolayers and synergizes with pro-inflammatory IL-1 |
| title_full_unstemmed | RSPO3 impairs barrier function of human vascular endothelial monolayers and synergizes with pro-inflammatory IL-1 |
| title_short | RSPO3 impairs barrier function of human vascular endothelial monolayers and synergizes with pro-inflammatory IL-1 |
| title_sort | rspo3 impairs barrier function of human vascular endothelial monolayers and synergizes with pro inflammatory il 1 |
| topic | Endothelial dysfunction RSPO3 Inflammation Vascular leakage IL-1β |
| url | http://link.springer.com/article/10.1186/s10020-018-0048-z |
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