Reciprocal mutations of lung-tropic AAV capsids lead to improved transduction properties
Considerable effort has been devoted to developing adeno-associated virus (AAV)-based vectors for gene therapy in cystic fibrosis (CF). As a result of directed evolution and capsid shuffling technology, AAV capsids are available with widespread tropism for airway epithelial cells. For example, AAV2....
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-11-01
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| Series: | Frontiers in Genome Editing |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgeed.2023.1271813/full |
| _version_ | 1797491352981733376 |
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| author | Ashley L. Cooney Ashley L. Cooney Ashley L. Cooney Christian M. Brommel Christian M. Brommel Christian M. Brommel Soumba Traore Soumba Traore Soumba Traore Gregory A. Newby Gregory A. Newby Gregory A. Newby David R. Liu David R. Liu David R. Liu Paul B. McCray Paul B. McCray Paul B. McCray Patrick L. Sinn Patrick L. Sinn Patrick L. Sinn |
| author_facet | Ashley L. Cooney Ashley L. Cooney Ashley L. Cooney Christian M. Brommel Christian M. Brommel Christian M. Brommel Soumba Traore Soumba Traore Soumba Traore Gregory A. Newby Gregory A. Newby Gregory A. Newby David R. Liu David R. Liu David R. Liu Paul B. McCray Paul B. McCray Paul B. McCray Patrick L. Sinn Patrick L. Sinn Patrick L. Sinn |
| author_sort | Ashley L. Cooney |
| collection | DOAJ |
| description | Considerable effort has been devoted to developing adeno-associated virus (AAV)-based vectors for gene therapy in cystic fibrosis (CF). As a result of directed evolution and capsid shuffling technology, AAV capsids are available with widespread tropism for airway epithelial cells. For example, AAV2.5T and AAV6.2 are two evolved capsids with improved airway epithelial cell transduction properties over their parental serotypes. However, limited research has been focused on identifying their specific cellular tropism. Restoring cystic fibrosis transmembrane conductance regulator (CFTR) expression in surface columnar epithelial cells is necessary for the correction of the CF airway phenotype. Basal cells are a progenitor population of the conducting airways responsible for replenishing surface epithelial cells (including secretory cells and ionocytes), making correction of this cell population vital for a long-lived gene therapy strategy. In this study, we investigate the tropism of AAV capsids for three cell types in primary cultures of well-differentiated human airway epithelial (HAE) cells and primary human airway basal cells. We observed that AAV2.5T transduced surface epithelial cells better than AAV6.2, while AAV6.2 transduced airway basal cells better than AAV2.5T. We also investigated a recently developed capsid, AAV6.2FF, which has two surface tyrosines converted to phenylalanines. Next, we incorporated reciprocal mutations to create AAV capsids with further improved surface and basal cell transduction characteristics. Lastly, we successfully employed a split-intein approach using AAV to deliver an adenine base editor (ABE) to repair the CFTRR553X mutation. Our results suggest that rational incorporation of AAV capsid mutations improves AAV transduction of the airway surface and progenitor cells and may ultimately lead to improved pulmonary function in people with CF. |
| first_indexed | 2024-03-10T00:47:11Z |
| format | Article |
| id | doaj.art-9022961518b6498c9920e9f7bf41f264 |
| institution | Directory Open Access Journal |
| issn | 2673-3439 |
| language | English |
| last_indexed | 2024-03-10T00:47:11Z |
| publishDate | 2023-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genome Editing |
| spelling | doaj.art-9022961518b6498c9920e9f7bf41f2642023-11-23T14:57:50ZengFrontiers Media S.A.Frontiers in Genome Editing2673-34392023-11-01510.3389/fgeed.2023.12718131271813Reciprocal mutations of lung-tropic AAV capsids lead to improved transduction propertiesAshley L. Cooney0Ashley L. Cooney1Ashley L. Cooney2Christian M. Brommel3Christian M. Brommel4Christian M. Brommel5Soumba Traore6Soumba Traore7Soumba Traore8Gregory A. Newby9Gregory A. Newby10Gregory A. Newby11David R. Liu12David R. Liu13David R. Liu14Paul B. McCray15Paul B. McCray16Paul B. McCray17Patrick L. Sinn18Patrick L. Sinn19Patrick L. Sinn20University of Iowa, Stead Family Department of Pediatrics, Iowa City, IA, United StatesPappajohn Biomedical Institute, Iowa City, IA, United StatesCenter for Cystic Fibrosis Gene Therapy, University of Iowa, Iowa City, IA, United StatesUniversity of Iowa, Stead Family Department of Pediatrics, Iowa City, IA, United StatesPappajohn Biomedical Institute, Iowa City, IA, United StatesCenter for Cystic Fibrosis Gene Therapy, University of Iowa, Iowa City, IA, United StatesUniversity of Iowa, Stead Family Department of Pediatrics, Iowa City, IA, United StatesPappajohn Biomedical Institute, Iowa City, IA, United StatesCenter for Cystic Fibrosis Gene Therapy, University of Iowa, Iowa City, IA, United StatesMerkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT, Cambridge, MA, United StatesDepartment of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, United StatesHoward Hughes Medical Institute, Harvard University, Cambridge, MA, United StatesMerkin Institute of Transformative Technologies in Healthcare, Broad Institute of Harvard and MIT, Cambridge, MA, United StatesDepartment of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, United StatesHoward Hughes Medical Institute, Harvard University, Cambridge, MA, United StatesUniversity of Iowa, Stead Family Department of Pediatrics, Iowa City, IA, United StatesPappajohn Biomedical Institute, Iowa City, IA, United StatesCenter for Cystic Fibrosis Gene Therapy, University of Iowa, Iowa City, IA, United StatesUniversity of Iowa, Stead Family Department of Pediatrics, Iowa City, IA, United StatesPappajohn Biomedical Institute, Iowa City, IA, United StatesCenter for Cystic Fibrosis Gene Therapy, University of Iowa, Iowa City, IA, United StatesConsiderable effort has been devoted to developing adeno-associated virus (AAV)-based vectors for gene therapy in cystic fibrosis (CF). As a result of directed evolution and capsid shuffling technology, AAV capsids are available with widespread tropism for airway epithelial cells. For example, AAV2.5T and AAV6.2 are two evolved capsids with improved airway epithelial cell transduction properties over their parental serotypes. However, limited research has been focused on identifying their specific cellular tropism. Restoring cystic fibrosis transmembrane conductance regulator (CFTR) expression in surface columnar epithelial cells is necessary for the correction of the CF airway phenotype. Basal cells are a progenitor population of the conducting airways responsible for replenishing surface epithelial cells (including secretory cells and ionocytes), making correction of this cell population vital for a long-lived gene therapy strategy. In this study, we investigate the tropism of AAV capsids for three cell types in primary cultures of well-differentiated human airway epithelial (HAE) cells and primary human airway basal cells. We observed that AAV2.5T transduced surface epithelial cells better than AAV6.2, while AAV6.2 transduced airway basal cells better than AAV2.5T. We also investigated a recently developed capsid, AAV6.2FF, which has two surface tyrosines converted to phenylalanines. Next, we incorporated reciprocal mutations to create AAV capsids with further improved surface and basal cell transduction characteristics. Lastly, we successfully employed a split-intein approach using AAV to deliver an adenine base editor (ABE) to repair the CFTRR553X mutation. Our results suggest that rational incorporation of AAV capsid mutations improves AAV transduction of the airway surface and progenitor cells and may ultimately lead to improved pulmonary function in people with CF.https://www.frontiersin.org/articles/10.3389/fgeed.2023.1271813/fulladeno-associated virus capsidgene therapycystic fibrosisairway epitheliabase editing |
| spellingShingle | Ashley L. Cooney Ashley L. Cooney Ashley L. Cooney Christian M. Brommel Christian M. Brommel Christian M. Brommel Soumba Traore Soumba Traore Soumba Traore Gregory A. Newby Gregory A. Newby Gregory A. Newby David R. Liu David R. Liu David R. Liu Paul B. McCray Paul B. McCray Paul B. McCray Patrick L. Sinn Patrick L. Sinn Patrick L. Sinn Reciprocal mutations of lung-tropic AAV capsids lead to improved transduction properties Frontiers in Genome Editing adeno-associated virus capsid gene therapy cystic fibrosis airway epithelia base editing |
| title | Reciprocal mutations of lung-tropic AAV capsids lead to improved transduction properties |
| title_full | Reciprocal mutations of lung-tropic AAV capsids lead to improved transduction properties |
| title_fullStr | Reciprocal mutations of lung-tropic AAV capsids lead to improved transduction properties |
| title_full_unstemmed | Reciprocal mutations of lung-tropic AAV capsids lead to improved transduction properties |
| title_short | Reciprocal mutations of lung-tropic AAV capsids lead to improved transduction properties |
| title_sort | reciprocal mutations of lung tropic aav capsids lead to improved transduction properties |
| topic | adeno-associated virus capsid gene therapy cystic fibrosis airway epithelia base editing |
| url | https://www.frontiersin.org/articles/10.3389/fgeed.2023.1271813/full |
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