An improved and cost-effective methodology for the reduction of autofluorescence in direct immunofluorescence studies on formalin-fixed paraffin-embedded tissues

Interference by autofluorescence is one of the major shortcomes of immunofluorescence analysis by confocal laser scanning microscopy (CLSM). CLSM requires minimal tissue autofluorescence and reduced unspecific fluorescence background, requisites that become more critical when direct immunofluorescen...

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Bibliographic Details
Main Authors: MS Viegas, TC Martins, F Seco, A do Carmo
Format: Article
Language:English
Published: PAGEPress Publications 2007-03-01
Series:European Journal of Histochemistry
Online Access:http://www.ejh.it/index.php/ejh/article/view/1013