A New Method to Characterize Conformation-Specific Antibody by a Combination of Agarose Native Gel Electrophoresis and Contact Blotting

A New Method to Characterize Conformation-Specific Antibody by a Combination of Agarose Native Gel Electrophoresis and Contact Blotting

In this study, we review the agarose native gel electrophoresis that separates proteins and macromolecular complexes in their native state and transfer of the separated proteins from the agarose gel to membranes by contact blotting which retains the native state of these structures. Green fluorescen...

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Bibliographic Details
Main Authors: Teruo Akuta, Toshiaki Maruyama, Chiaki Sakuma, Masataka Nakagawa, Yui Tomioka, Kevin Entzminger, Jonathan K. Fleming, Ryo Sato, Takashi Shibata, Yasunori Kurosawa, C. J. Okumura, Tsutomu Arakawa
Format: Article
Language:English
Published: MDPI AG 2022-05-01
Series:Antibodies
Subjects:
native gel
blotting
conformation-specific
antibody
SARS-CoV-2
conformation-indifferential
Online Access:https://www.mdpi.com/2073-4468/11/2/36

Internet

https://www.mdpi.com/2073-4468/11/2/36

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