Profiling Thermus thermophilus Argonaute Guide DNA Sequence Preferences by Functional Screening

Prokaryotic Argonautes (pAgo) are an increasingly well-studied class of guided endonucleases, and the underlying mechanisms by which pAgo generate nucleic acid guides in vivo remains an important topic of investigation. Recent insights into these mechanisms for the Argonaute protein from Thermus the...

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Bibliographic Details
Main Authors: Eric A. Hunt, Esta Tamanaha, Kevin Bonanno, Eric J. Cantor, Nathan A. Tanner
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-04-01
Series:Frontiers in Molecular Biosciences
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fmolb.2021.670940/full