Preparation and structure characterization of a natural acetylated fructooligosaccharide from Polygonatum sibiricum and its alleviative effect on colitis by inhibiting NLRP3 pathway
This study aimed to isolate oligosaccharides from P. sibiricum, analyze its structure, and elucidate the pharmacological mechanism for colitis improvement. First, a homogeneous oligosaccharide PSO-A was isolated and its structure was characterized. Then, the colitis model was induced with 3 % dextra...
| Päätekijät: | , , , , , , , , , |
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| Aineistotyyppi: | Artikkeli |
| Kieli: | English |
| Julkaistu: |
Elsevier
2024-02-01
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| Sarja: | Journal of Functional Foods |
| Aiheet: | |
| Linkit: | http://www.sciencedirect.com/science/article/pii/S175646462400029X |
| _version_ | 1827351349005647872 |
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| author | Shengchen Ding Jun Chen Guangming Chen Weiyi Tian Junping Zheng Haiming Hu Lu Cao Xiaowei Yao Baifei Hu Hongtao Liu |
| author_facet | Shengchen Ding Jun Chen Guangming Chen Weiyi Tian Junping Zheng Haiming Hu Lu Cao Xiaowei Yao Baifei Hu Hongtao Liu |
| author_sort | Shengchen Ding |
| collection | DOAJ |
| description | This study aimed to isolate oligosaccharides from P. sibiricum, analyze its structure, and elucidate the pharmacological mechanism for colitis improvement. First, a homogeneous oligosaccharide PSO-A was isolated and its structure was characterized. Then, the colitis model was induced with 3 % dextran sulfate sodium (DSS) and intervened with PSO-A. The results show that PSO-A was an agavin-type acetylated fructooligosaccharide with a molecular weight of 2.12 kDa. PSO-A consisted of (2 → 6)-β-D-Fruf residue backbone with an internal α-D-Glcp residue and (2 → 1)-β-D-Fruf residue side chains. Further, PSO-A increased mucin content, protected intestinal barrier integrity of colitis mice, and significantly inhibited the levels of inflammatory factors. Moreover, the protein expressions of NLRP3, ASC, and Caspase-1 in colitis mice was statistically inhibited by PSO-A. In summary, PSO-A could alleviate colitis by inhibiting NLRP3 inflammasome pathway, which provides a basis for the development and application of P. sibiricum oligosaccharides as drug or functional food to relieve colitis. |
| first_indexed | 2024-03-08T02:02:14Z |
| format | Article |
| id | doaj.art-90974c30c7f440248048e24f041ef38c |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-03-08T02:02:14Z |
| publishDate | 2024-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-90974c30c7f440248048e24f041ef38c2024-02-14T05:14:31ZengElsevierJournal of Functional Foods1756-46462024-02-01113106027Preparation and structure characterization of a natural acetylated fructooligosaccharide from Polygonatum sibiricum and its alleviative effect on colitis by inhibiting NLRP3 pathwayShengchen Ding0Jun Chen1Guangming Chen2Weiyi Tian3Junping Zheng4Haiming Hu5Lu Cao6Xiaowei Yao7Baifei Hu8Hongtao Liu9School of Basic Medical Sciences, Hubei University of Chinese Medicine, Huangjiahu West Road 16, Wuhan 430065, PR China; School of Pharmacy, Hubei University of Chinese Medicine, Huangjiahu West Road 16, Wuhan 430065, PR ChinaSchool of Basic Medical Sciences, Hubei University of Chinese Medicine, Huangjiahu West Road 16, Wuhan 430065, PR China; School of Pharmacy, Hubei University of Chinese Medicine, Huangjiahu West Road 16, Wuhan 430065, PR ChinaSchool of Basic Medical Sciences, Hubei University of Chinese Medicine, Huangjiahu West Road 16, Wuhan 430065, PR ChinaCollege of Basic Medical Sciences, Guizhou University of Traditional Chinese Medicine, Dongqing Road 4, Guiyang 550025, PR ChinaSchool of Basic Medical Sciences, Hubei University of Chinese Medicine, Huangjiahu West Road 16, Wuhan 430065, PR ChinaSchool of Laboratory Medicine, Hubei University of Chinese Medicine, Huangjiahu West Road 16, Wuhan 430065, PR ChinaSchool of Basic Medical Sciences, Hubei University of Chinese Medicine, Huangjiahu West Road 16, Wuhan 430065, PR ChinaSchool of Basic Medical Sciences, Hubei University of Chinese Medicine, Huangjiahu West Road 16, Wuhan 430065, PR ChinaSchool of Basic Medical Sciences, Hubei University of Chinese Medicine, Huangjiahu West Road 16, Wuhan 430065, PR China; Corresponding authors at: School of Basic Medical Sciences, Hubei University of Chinese Medicine, Huangjiahu West Road 16, Wuhan 430065, PR China (H. Liu).School of Basic Medical Sciences, Hubei University of Chinese Medicine, Huangjiahu West Road 16, Wuhan 430065, PR China; School of Pharmacy, Hubei University of Chinese Medicine, Huangjiahu West Road 16, Wuhan 430065, PR China; Corresponding authors at: School of Basic Medical Sciences, Hubei University of Chinese Medicine, Huangjiahu West Road 16, Wuhan 430065, PR China (H. Liu).This study aimed to isolate oligosaccharides from P. sibiricum, analyze its structure, and elucidate the pharmacological mechanism for colitis improvement. First, a homogeneous oligosaccharide PSO-A was isolated and its structure was characterized. Then, the colitis model was induced with 3 % dextran sulfate sodium (DSS) and intervened with PSO-A. The results show that PSO-A was an agavin-type acetylated fructooligosaccharide with a molecular weight of 2.12 kDa. PSO-A consisted of (2 → 6)-β-D-Fruf residue backbone with an internal α-D-Glcp residue and (2 → 1)-β-D-Fruf residue side chains. Further, PSO-A increased mucin content, protected intestinal barrier integrity of colitis mice, and significantly inhibited the levels of inflammatory factors. Moreover, the protein expressions of NLRP3, ASC, and Caspase-1 in colitis mice was statistically inhibited by PSO-A. In summary, PSO-A could alleviate colitis by inhibiting NLRP3 inflammasome pathway, which provides a basis for the development and application of P. sibiricum oligosaccharides as drug or functional food to relieve colitis.http://www.sciencedirect.com/science/article/pii/S175646462400029XPolygonatum sibiricumOligosaccharideCharacterizationIntestinal inflammationColitisInflammasome |
| spellingShingle | Shengchen Ding Jun Chen Guangming Chen Weiyi Tian Junping Zheng Haiming Hu Lu Cao Xiaowei Yao Baifei Hu Hongtao Liu Preparation and structure characterization of a natural acetylated fructooligosaccharide from Polygonatum sibiricum and its alleviative effect on colitis by inhibiting NLRP3 pathway Journal of Functional Foods Polygonatum sibiricum Oligosaccharide Characterization Intestinal inflammation Colitis Inflammasome |
| title | Preparation and structure characterization of a natural acetylated fructooligosaccharide from Polygonatum sibiricum and its alleviative effect on colitis by inhibiting NLRP3 pathway |
| title_full | Preparation and structure characterization of a natural acetylated fructooligosaccharide from Polygonatum sibiricum and its alleviative effect on colitis by inhibiting NLRP3 pathway |
| title_fullStr | Preparation and structure characterization of a natural acetylated fructooligosaccharide from Polygonatum sibiricum and its alleviative effect on colitis by inhibiting NLRP3 pathway |
| title_full_unstemmed | Preparation and structure characterization of a natural acetylated fructooligosaccharide from Polygonatum sibiricum and its alleviative effect on colitis by inhibiting NLRP3 pathway |
| title_short | Preparation and structure characterization of a natural acetylated fructooligosaccharide from Polygonatum sibiricum and its alleviative effect on colitis by inhibiting NLRP3 pathway |
| title_sort | preparation and structure characterization of a natural acetylated fructooligosaccharide from polygonatum sibiricum and its alleviative effect on colitis by inhibiting nlrp3 pathway |
| topic | Polygonatum sibiricum Oligosaccharide Characterization Intestinal inflammation Colitis Inflammasome |
| url | http://www.sciencedirect.com/science/article/pii/S175646462400029X |
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