Polysome profiling followed by quantitative PCR for identifying potential micropeptide encoding long non-coding RNAs in suspension cell lines

Summary: Micropeptides are emerging as important regulators of various cellular processes. Long non-coding RNAs (lncRNAs) serve as a source of micropeptide-encoding small reading frames. The techniques to detect micropeptides or translating lncRNAs, such as mass spectrometry and ribosome profiling,...

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Bibliographic Details
Main Authors: Cai Han, Linyu Sun, Qi Pan, Yumeng Sun, Wentao Wang, Yueqin Chen
Format: Article
Language:English
Published: Elsevier 2022-03-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721007437