Polysome profiling followed by quantitative PCR for identifying potential micropeptide encoding long non-coding RNAs in suspension cell lines
Summary: Micropeptides are emerging as important regulators of various cellular processes. Long non-coding RNAs (lncRNAs) serve as a source of micropeptide-encoding small reading frames. The techniques to detect micropeptides or translating lncRNAs, such as mass spectrometry and ribosome profiling,...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-03-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721007437 |
| _version_ | 1818318881954988032 |
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| author | Cai Han Linyu Sun Qi Pan Yumeng Sun Wentao Wang Yueqin Chen |
| author_facet | Cai Han Linyu Sun Qi Pan Yumeng Sun Wentao Wang Yueqin Chen |
| author_sort | Cai Han |
| collection | DOAJ |
| description | Summary: Micropeptides are emerging as important regulators of various cellular processes. Long non-coding RNAs (lncRNAs) serve as a source of micropeptide-encoding small reading frames. The techniques to detect micropeptides or translating lncRNAs, such as mass spectrometry and ribosome profiling, are sophisticated and expensive. Here, we present an easy and cost-effective protocol to screen for potential micropeptide-encoding lncRNAs by polysome profiling in suspension cell lines. When combined with quantitative PCR, this protocol facilitates the identification of a number of translating lncRNAs simultaneously.For complete details on the use and execution of this protocol, please refer to Sun et al. (2021). |
| first_indexed | 2024-12-13T10:00:16Z |
| format | Article |
| id | doaj.art-909b3fff03214ca3946820dc63b00298 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-13T10:00:16Z |
| publishDate | 2022-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-909b3fff03214ca3946820dc63b002982022-12-21T23:51:41ZengElsevierSTAR Protocols2666-16672022-03-0131101037Polysome profiling followed by quantitative PCR for identifying potential micropeptide encoding long non-coding RNAs in suspension cell linesCai Han0Linyu Sun1Qi Pan2Yumeng Sun3Wentao Wang4Yueqin Chen5MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, Guangdong 510275, China; Corresponding authorMOE Key Laboratory of Gene Function and Regulation, State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, Guangdong 510275, ChinaMOE Key Laboratory of Gene Function and Regulation, State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, Guangdong 510275, ChinaMOE Key Laboratory of Gene Function and Regulation, State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, Guangdong 510275, ChinaMOE Key Laboratory of Gene Function and Regulation, State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, Guangdong 510275, ChinaMOE Key Laboratory of Gene Function and Regulation, State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, Guangdong 510275, China; Corresponding authorSummary: Micropeptides are emerging as important regulators of various cellular processes. Long non-coding RNAs (lncRNAs) serve as a source of micropeptide-encoding small reading frames. The techniques to detect micropeptides or translating lncRNAs, such as mass spectrometry and ribosome profiling, are sophisticated and expensive. Here, we present an easy and cost-effective protocol to screen for potential micropeptide-encoding lncRNAs by polysome profiling in suspension cell lines. When combined with quantitative PCR, this protocol facilitates the identification of a number of translating lncRNAs simultaneously.For complete details on the use and execution of this protocol, please refer to Sun et al. (2021).http://www.sciencedirect.com/science/article/pii/S2666166721007437Cell separation/fractionationMolecular Biology |
| spellingShingle | Cai Han Linyu Sun Qi Pan Yumeng Sun Wentao Wang Yueqin Chen Polysome profiling followed by quantitative PCR for identifying potential micropeptide encoding long non-coding RNAs in suspension cell lines STAR Protocols Cell separation/fractionation Molecular Biology |
| title | Polysome profiling followed by quantitative PCR for identifying potential micropeptide encoding long non-coding RNAs in suspension cell lines |
| title_full | Polysome profiling followed by quantitative PCR for identifying potential micropeptide encoding long non-coding RNAs in suspension cell lines |
| title_fullStr | Polysome profiling followed by quantitative PCR for identifying potential micropeptide encoding long non-coding RNAs in suspension cell lines |
| title_full_unstemmed | Polysome profiling followed by quantitative PCR for identifying potential micropeptide encoding long non-coding RNAs in suspension cell lines |
| title_short | Polysome profiling followed by quantitative PCR for identifying potential micropeptide encoding long non-coding RNAs in suspension cell lines |
| title_sort | polysome profiling followed by quantitative pcr for identifying potential micropeptide encoding long non coding rnas in suspension cell lines |
| topic | Cell separation/fractionation Molecular Biology |
| url | http://www.sciencedirect.com/science/article/pii/S2666166721007437 |
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