Adaptive phase contrast microscopy to compensate for the meniscus effect

Abstract Phase contrast is one of the most important microscopic methods for making visible transparent, unstained cells. Cell cultures are often cultivated in microtiter plates, consisting of several cylindrical wells. The surface tension of the culture medium forms a liquid lens within the well, c...

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Bibliographic Details
Main Authors: Florian Nienhaus, Tobias Piotrowski, Bastian Nießing, Niels König, Robert H. Schmitt
Format: Article
Language:English
Published: Nature Portfolio 2023-04-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-023-32917-6