Microarray-based genotyping and clinical outcomes of Staphylococcus aureus bloodstream infection: an exploratory study.
The clinical course of Staphylococcus aureus bacteremia varies extensively. We sought to determine the relationship between genetic characteristics of the infecting pathogen and clinical outcomes in an exploratory study. In two study centers, 317 blood culture isolates were analyzed by DNA microarra...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23967176/pdf/?tool=EBI |
| _version_ | 1828897306618888192 |
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| author | Siegbert Rieg Daniel Jonas Achim J Kaasch Christine Porzelius Gabriele Peyerl-Hoffmann Christian Theilacker Marc-Fabian Küpper Christian Schneider Harald Seifert Winfried V Kern |
| author_facet | Siegbert Rieg Daniel Jonas Achim J Kaasch Christine Porzelius Gabriele Peyerl-Hoffmann Christian Theilacker Marc-Fabian Küpper Christian Schneider Harald Seifert Winfried V Kern |
| author_sort | Siegbert Rieg |
| collection | DOAJ |
| description | The clinical course of Staphylococcus aureus bacteremia varies extensively. We sought to determine the relationship between genetic characteristics of the infecting pathogen and clinical outcomes in an exploratory study. In two study centers, 317 blood culture isolates were analyzed by DNA microarray and spa genotyping. By uni- and multivariate regression analyses associations of genotype data with 30-day all-cause mortality, severe sepsis/septic shock, disseminated disease, endocarditis, and osteoarticular infection were investigated. Univariate analysis showed significant association between S. aureus genes/gene-clusters or clonal complexes and clinical endpoints. For example CC15 was associated with 30-day mortality and CC22 with osteoarticular infection. In multivariate analysis methicillin resistance (mecA, OR 4.8 [1.43-16.06]) and the beta-lactamase-gene (bla, OR 3.12 [1.17-8.30]) remained independently associated with 30-day mortality. The presence of genes for enterotoxins (sed/sej/ser) was associated with endocarditis (OR 5.11 [1.14-18.62]). Host factors such as McCabe classification (OR 4.52 [2.09-9.79] for mortality), age (OR 1.06 [1.03-1.10] per year), and community-acquisition (OR 3.40 [1.31-8.81]) had a major influence on disease severity, dissemination and mortality. Individual genotypes and clonal complexes of S. aureus can only partially explain clinical features and outcomes of S. aureus bacteremia. Genotype-phenotype association studies need to include adjustments for host factors like age, comorbidity and community-acquisition. |
| first_indexed | 2024-12-13T14:54:08Z |
| format | Article |
| id | doaj.art-90a6399ad61e452db29bb3d72fc0b851 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T14:54:08Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-90a6399ad61e452db29bb3d72fc0b8512022-12-21T23:41:16ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0188e7125910.1371/journal.pone.0071259Microarray-based genotyping and clinical outcomes of Staphylococcus aureus bloodstream infection: an exploratory study.Siegbert RiegDaniel JonasAchim J KaaschChristine PorzeliusGabriele Peyerl-HoffmannChristian TheilackerMarc-Fabian KüpperChristian SchneiderHarald SeifertWinfried V KernThe clinical course of Staphylococcus aureus bacteremia varies extensively. We sought to determine the relationship between genetic characteristics of the infecting pathogen and clinical outcomes in an exploratory study. In two study centers, 317 blood culture isolates were analyzed by DNA microarray and spa genotyping. By uni- and multivariate regression analyses associations of genotype data with 30-day all-cause mortality, severe sepsis/septic shock, disseminated disease, endocarditis, and osteoarticular infection were investigated. Univariate analysis showed significant association between S. aureus genes/gene-clusters or clonal complexes and clinical endpoints. For example CC15 was associated with 30-day mortality and CC22 with osteoarticular infection. In multivariate analysis methicillin resistance (mecA, OR 4.8 [1.43-16.06]) and the beta-lactamase-gene (bla, OR 3.12 [1.17-8.30]) remained independently associated with 30-day mortality. The presence of genes for enterotoxins (sed/sej/ser) was associated with endocarditis (OR 5.11 [1.14-18.62]). Host factors such as McCabe classification (OR 4.52 [2.09-9.79] for mortality), age (OR 1.06 [1.03-1.10] per year), and community-acquisition (OR 3.40 [1.31-8.81]) had a major influence on disease severity, dissemination and mortality. Individual genotypes and clonal complexes of S. aureus can only partially explain clinical features and outcomes of S. aureus bacteremia. Genotype-phenotype association studies need to include adjustments for host factors like age, comorbidity and community-acquisition.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23967176/pdf/?tool=EBI |
| spellingShingle | Siegbert Rieg Daniel Jonas Achim J Kaasch Christine Porzelius Gabriele Peyerl-Hoffmann Christian Theilacker Marc-Fabian Küpper Christian Schneider Harald Seifert Winfried V Kern Microarray-based genotyping and clinical outcomes of Staphylococcus aureus bloodstream infection: an exploratory study. PLoS ONE |
| title | Microarray-based genotyping and clinical outcomes of Staphylococcus aureus bloodstream infection: an exploratory study. |
| title_full | Microarray-based genotyping and clinical outcomes of Staphylococcus aureus bloodstream infection: an exploratory study. |
| title_fullStr | Microarray-based genotyping and clinical outcomes of Staphylococcus aureus bloodstream infection: an exploratory study. |
| title_full_unstemmed | Microarray-based genotyping and clinical outcomes of Staphylococcus aureus bloodstream infection: an exploratory study. |
| title_short | Microarray-based genotyping and clinical outcomes of Staphylococcus aureus bloodstream infection: an exploratory study. |
| title_sort | microarray based genotyping and clinical outcomes of staphylococcus aureus bloodstream infection an exploratory study |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23967176/pdf/?tool=EBI |
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