Antibiotic resistance in potential probiotic lactic acid bacteria of fermented foods and human origin from Nigeria
Abstract Introduction Probiotic lactobacilli are generally recognized as safe (GRAS) and are being used in several food and pharma formulations. However, growing concern of antibiotic resistance in bacterial strains of food origin and its possible transmission via functional foods is increasingly be...
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| Format: | Article |
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BMC
2023-05-01
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| Series: | BMC Microbiology |
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| Online Access: | https://doi.org/10.1186/s12866-023-02883-0 |
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| author | Rachael T. Duche Anamika Singh Arundhati Ganesh Wandhare Vikas Sangwan Manvesh Kumar Sihag Tochukwu N. T. Nwagu Harsh Panwar Lewis. I. Ezeogu |
| author_facet | Rachael T. Duche Anamika Singh Arundhati Ganesh Wandhare Vikas Sangwan Manvesh Kumar Sihag Tochukwu N. T. Nwagu Harsh Panwar Lewis. I. Ezeogu |
| author_sort | Rachael T. Duche |
| collection | DOAJ |
| description | Abstract Introduction Probiotic lactobacilli are generally recognized as safe (GRAS) and are being used in several food and pharma formulations. However, growing concern of antibiotic resistance in bacterial strains of food origin and its possible transmission via functional foods is increasingly being emphasized. Objectives This study screened potential probiotic lactic acid bacteria (LAB) strains for their phenotypic and genotypic antibiotic resistance profiles. Methods Susceptibility to different antibiotics was assayed by the Kirby Bauer standard disc diffusion protocol. Both conventional and SYBR-RTq-PCR were used for detection of resistance coding genes. Results A variable susceptibility pattern was documented against different antibiotic classes. LAB strains irrespective of origin displayed marked phenotypic resistance against cephalosporins, aminoglycosides, quinolones, glycopeptides; and methicillin among beta-lactams with few exceptions. In contrast, high sensitivity was recorded against macrolides, sulphonamides and carbapenems sub-group of beta-lactams with some variations. parC, associated with ciprofloxacin resistance was detected in 76.5% of the strains. Other prevalent resistant determinants observed were aac(6?)Ii (42.1%), ermB, ermC (29.4%), and tetM (20.5%). Six (?17.6%) of the isolates were free from genetic resistance determinants screened in this study. Conclusion Study revealed presence of antibiotic resistance determinants among lactobacilli from both fermented foods and human sources. |
| first_indexed | 2024-03-13T10:16:55Z |
| format | Article |
| id | doaj.art-90c5f306b9294cd28786efb619e08259 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-03-13T10:16:55Z |
| publishDate | 2023-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-90c5f306b9294cd28786efb619e082592023-05-21T11:10:15ZengBMCBMC Microbiology1471-21802023-05-0123111810.1186/s12866-023-02883-0Antibiotic resistance in potential probiotic lactic acid bacteria of fermented foods and human origin from NigeriaRachael T. Duche0Anamika Singh1Arundhati Ganesh Wandhare2Vikas Sangwan3Manvesh Kumar Sihag4Tochukwu N. T. Nwagu5Harsh Panwar6Lewis. I. Ezeogu7Department of Dairy Microbiology, Guru Angad Dev Veterinary and Animal Sciences UniversityDepartment of Dairy Microbiology, Guru Angad Dev Veterinary and Animal Sciences UniversityDepartment of Dairy Microbiology, Guru Angad Dev Veterinary and Animal Sciences UniversityDepartment of Dairy Microbiology, Guru Angad Dev Veterinary and Animal Sciences UniversityDepartment of Dairy Chemistry, Guru Angad Dev Veterinary and Animal Sciences UniversityDepartment of Microbiology, University of Nigeria NsukkaDepartment of Dairy Microbiology, Guru Angad Dev Veterinary and Animal Sciences UniversityDepartment of Microbiology, University of Nigeria NsukkaAbstract Introduction Probiotic lactobacilli are generally recognized as safe (GRAS) and are being used in several food and pharma formulations. However, growing concern of antibiotic resistance in bacterial strains of food origin and its possible transmission via functional foods is increasingly being emphasized. Objectives This study screened potential probiotic lactic acid bacteria (LAB) strains for their phenotypic and genotypic antibiotic resistance profiles. Methods Susceptibility to different antibiotics was assayed by the Kirby Bauer standard disc diffusion protocol. Both conventional and SYBR-RTq-PCR were used for detection of resistance coding genes. Results A variable susceptibility pattern was documented against different antibiotic classes. LAB strains irrespective of origin displayed marked phenotypic resistance against cephalosporins, aminoglycosides, quinolones, glycopeptides; and methicillin among beta-lactams with few exceptions. In contrast, high sensitivity was recorded against macrolides, sulphonamides and carbapenems sub-group of beta-lactams with some variations. parC, associated with ciprofloxacin resistance was detected in 76.5% of the strains. Other prevalent resistant determinants observed were aac(6?)Ii (42.1%), ermB, ermC (29.4%), and tetM (20.5%). Six (?17.6%) of the isolates were free from genetic resistance determinants screened in this study. Conclusion Study revealed presence of antibiotic resistance determinants among lactobacilli from both fermented foods and human sources.https://doi.org/10.1186/s12866-023-02883-0Antibiotic resistanceLactic acid bacteriaLactobacilliMulti drug resistantResistance genes |
| spellingShingle | Rachael T. Duche Anamika Singh Arundhati Ganesh Wandhare Vikas Sangwan Manvesh Kumar Sihag Tochukwu N. T. Nwagu Harsh Panwar Lewis. I. Ezeogu Antibiotic resistance in potential probiotic lactic acid bacteria of fermented foods and human origin from Nigeria BMC Microbiology Antibiotic resistance Lactic acid bacteria Lactobacilli Multi drug resistant Resistance genes |
| title | Antibiotic resistance in potential probiotic lactic acid bacteria of fermented foods and human origin from Nigeria |
| title_full | Antibiotic resistance in potential probiotic lactic acid bacteria of fermented foods and human origin from Nigeria |
| title_fullStr | Antibiotic resistance in potential probiotic lactic acid bacteria of fermented foods and human origin from Nigeria |
| title_full_unstemmed | Antibiotic resistance in potential probiotic lactic acid bacteria of fermented foods and human origin from Nigeria |
| title_short | Antibiotic resistance in potential probiotic lactic acid bacteria of fermented foods and human origin from Nigeria |
| title_sort | antibiotic resistance in potential probiotic lactic acid bacteria of fermented foods and human origin from nigeria |
| topic | Antibiotic resistance Lactic acid bacteria Lactobacilli Multi drug resistant Resistance genes |
| url | https://doi.org/10.1186/s12866-023-02883-0 |
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