Generation and validation of a Shewanella oneidensis MR-1 clone set for protein expression and phage display.

Generation and validation of a Shewanella oneidensis MR-1 clone set for protein expression and phage display.

A comprehensive gene collection for S. oneidensis was constructed using the lambda recombinase (Gateway) cloning system. A total of 3584 individual ORFs (85%) have been successfully cloned into the entry plasmids. To validate the use of the clone set, three sets of ORFs were examined within three di...

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Bibliographic Details
Main Authors: Haichun Gao, Donna Pattison, Tingfen Yan, Dawn M Klingeman, Xiaohu Wang, Joseph Petrosino, Lisa Hemphill, Xiufeng Wan, Adam B Leaphart, George M Weinstock, Timothy Palzkill, Jizhong Zhou
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2008-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC2500165?pdf=render

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http://europepmc.org/articles/PMC2500165?pdf=render

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