Exploring the phenolic profile, antibacterial, and antioxidant properties of walnut leaves (Juglans regia L.)
Abstract The aim of this study was to identify phenolic compounds in walnut leaves from northern Iraq and evaluate their ability to act as antibacterial and antioxidant agents. Phenolic compounds were determined by reversed‐phase HPLC. Antibacterial activity was tested against various bacteria. Anti...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-11-01
|
| Series: | Food Science & Nutrition |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/fsn3.3554 |
| _version_ | 1797633813260533760 |
|---|---|
| author | Ammar B. Altemimi Siba Mouid Al‐haliem Zina T. Alkanan Muthanna J. Mohammed Mohammad Ali Hesarinejad Mazin A. A. Najm Aziz Bouymajane Francesco Cacciola Tarek Gamal Abedelmaksoud |
| author_facet | Ammar B. Altemimi Siba Mouid Al‐haliem Zina T. Alkanan Muthanna J. Mohammed Mohammad Ali Hesarinejad Mazin A. A. Najm Aziz Bouymajane Francesco Cacciola Tarek Gamal Abedelmaksoud |
| author_sort | Ammar B. Altemimi |
| collection | DOAJ |
| description | Abstract The aim of this study was to identify phenolic compounds in walnut leaves from northern Iraq and evaluate their ability to act as antibacterial and antioxidant agents. Phenolic compounds were determined by reversed‐phase HPLC. Antibacterial activity was tested against various bacteria. Antioxidant properties were evaluated by various assays, including reducing power and DPPH radical scavenging activity. The HPLC profiles of walnut leaf fractions revealed quercetin, hydroquinone, 4‐hydroxybenzoic acid, and caffeic acid in three fractions. The inhibitory activity of DPPH was determined as 47.66, 32.41, and 51.90 μg/mL for fractions I, II, and III, respectively. For ferric reducing power activity, fraction II > fraction III > fraction I and the FRAP activity was observed as 64.43, 73.19, and 68.18 μg/mL for fractions I, II, and III, respectively. All extracted fractions had antibacterial properties against all bacterial strains tested. Observations showed that fraction I was able to produce similar zones of inhibition as streptomycin in most cases. These results suggest that the fractions of this plant extract are plausible natural antioxidants that could be used as prime candidates for the synthesis of antioxidant drugs that can be used for the treatment of many oxidative stress‐related diseases. |
| first_indexed | 2024-03-11T11:58:53Z |
| format | Article |
| id | doaj.art-911dedd1f42d43109ecb9b9cf588043d |
| institution | Directory Open Access Journal |
| issn | 2048-7177 |
| language | English |
| last_indexed | 2024-03-11T11:58:53Z |
| publishDate | 2023-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Food Science & Nutrition |
| spelling | doaj.art-911dedd1f42d43109ecb9b9cf588043d2023-11-08T09:39:18ZengWileyFood Science & Nutrition2048-71772023-11-0111116845685310.1002/fsn3.3554Exploring the phenolic profile, antibacterial, and antioxidant properties of walnut leaves (Juglans regia L.)Ammar B. Altemimi0Siba Mouid Al‐haliem1Zina T. Alkanan2Muthanna J. Mohammed3Mohammad Ali Hesarinejad4Mazin A. A. Najm5Aziz Bouymajane6Francesco Cacciola7Tarek Gamal Abedelmaksoud8Department of Food Science, College of Agriculture University of Basrah Basrah IraqDepartment of Dental Basic Sciences, College of Dentistry University of Mosul Mosul IraqDepartment of Food Science, College of Agriculture University of Basrah Basrah IraqDepartment of Biology, College of Education for Pure Sciences University of Mosul Mosul IraqDepartment of Food Processing Research Institute of Food Science and Technology Mashhad IranPharmaceutical chemistry department, College of Pharmacy Al‐Ayen University Thi‐Qar IraqTeam of Microbiology and Health, Laboratory of Chemistry‐Biology Applied to the Environment, Faculty of Sciences Moulay Ismail University Meknes MoroccoDepartment of Biomedical, Dental, Morphological and Functional Imaging Sciences University of Messina Messina ItalyFood Science Department, Faculty of Agriculture Cairo University Giza EgyptAbstract The aim of this study was to identify phenolic compounds in walnut leaves from northern Iraq and evaluate their ability to act as antibacterial and antioxidant agents. Phenolic compounds were determined by reversed‐phase HPLC. Antibacterial activity was tested against various bacteria. Antioxidant properties were evaluated by various assays, including reducing power and DPPH radical scavenging activity. The HPLC profiles of walnut leaf fractions revealed quercetin, hydroquinone, 4‐hydroxybenzoic acid, and caffeic acid in three fractions. The inhibitory activity of DPPH was determined as 47.66, 32.41, and 51.90 μg/mL for fractions I, II, and III, respectively. For ferric reducing power activity, fraction II > fraction III > fraction I and the FRAP activity was observed as 64.43, 73.19, and 68.18 μg/mL for fractions I, II, and III, respectively. All extracted fractions had antibacterial properties against all bacterial strains tested. Observations showed that fraction I was able to produce similar zones of inhibition as streptomycin in most cases. These results suggest that the fractions of this plant extract are plausible natural antioxidants that could be used as prime candidates for the synthesis of antioxidant drugs that can be used for the treatment of many oxidative stress‐related diseases.https://doi.org/10.1002/fsn3.3554caffeic acidhydroquinonequercetinwalnut |
| spellingShingle | Ammar B. Altemimi Siba Mouid Al‐haliem Zina T. Alkanan Muthanna J. Mohammed Mohammad Ali Hesarinejad Mazin A. A. Najm Aziz Bouymajane Francesco Cacciola Tarek Gamal Abedelmaksoud Exploring the phenolic profile, antibacterial, and antioxidant properties of walnut leaves (Juglans regia L.) Food Science & Nutrition caffeic acid hydroquinone quercetin walnut |
| title | Exploring the phenolic profile, antibacterial, and antioxidant properties of walnut leaves (Juglans regia L.) |
| title_full | Exploring the phenolic profile, antibacterial, and antioxidant properties of walnut leaves (Juglans regia L.) |
| title_fullStr | Exploring the phenolic profile, antibacterial, and antioxidant properties of walnut leaves (Juglans regia L.) |
| title_full_unstemmed | Exploring the phenolic profile, antibacterial, and antioxidant properties of walnut leaves (Juglans regia L.) |
| title_short | Exploring the phenolic profile, antibacterial, and antioxidant properties of walnut leaves (Juglans regia L.) |
| title_sort | exploring the phenolic profile antibacterial and antioxidant properties of walnut leaves juglans regia l |
| topic | caffeic acid hydroquinone quercetin walnut |
| url | https://doi.org/10.1002/fsn3.3554 |
| work_keys_str_mv | AT ammarbaltemimi exploringthephenolicprofileantibacterialandantioxidantpropertiesofwalnutleavesjuglansregial AT sibamouidalhaliem exploringthephenolicprofileantibacterialandantioxidantpropertiesofwalnutleavesjuglansregial AT zinatalkanan exploringthephenolicprofileantibacterialandantioxidantpropertiesofwalnutleavesjuglansregial AT muthannajmohammed exploringthephenolicprofileantibacterialandantioxidantpropertiesofwalnutleavesjuglansregial AT mohammadalihesarinejad exploringthephenolicprofileantibacterialandantioxidantpropertiesofwalnutleavesjuglansregial AT mazinaanajm exploringthephenolicprofileantibacterialandantioxidantpropertiesofwalnutleavesjuglansregial AT azizbouymajane exploringthephenolicprofileantibacterialandantioxidantpropertiesofwalnutleavesjuglansregial AT francescocacciola exploringthephenolicprofileantibacterialandantioxidantpropertiesofwalnutleavesjuglansregial AT tarekgamalabedelmaksoud exploringthephenolicprofileantibacterialandantioxidantpropertiesofwalnutleavesjuglansregial |