Quantitative Evaluation of Very Low Levels of HIV-1 Reverse Transcriptase by a Novel Highly Sensitive RT-qPCR Assay

Quantitative Evaluation of Very Low Levels of HIV-1 Reverse Transcriptase by a Novel Highly Sensitive RT-qPCR Assay

Based on previous experience in our laboratory, we developed a real-time reverse transcriptase (RT) quantitative PCR (RT-qPCR) assay for the assessment of very low levels of HIV-1 RT activity. The RNA, acting as a template for reverse transcription into cDNA by HIV-1 RT, consisted of a synthetic RNA...

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Bibliographic Details
Main Authors: Francesca Marino-Merlo, Valeria Stefanizzi, Agnese Ragno, Lucia Piredda, Sandro Grelli, Beatrice Macchi, Antonio Mastino
Format: Article
Language:English
Published: MDPI AG 2022-07-01
Series:Life
Subjects:
human immunodeficiency virus
reverse transcriptase
in vitro transcription
quantitative PCR assay
Online Access:https://www.mdpi.com/2075-1729/12/8/1130

Internet

https://www.mdpi.com/2075-1729/12/8/1130

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