Two-color nanoscopy of organelles for extended times with HIDE probes
High density environmentally sensitive (HIDE) probes allow for long time-lapse super-resolution imaging of live cells. Here the authors develop a second HIDE probe with a bio-orthogonal labelling strategy to enable two-color nanoscopy of two organelles over extended periods.
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2020-08-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-020-17859-1 |
| _version_ | 1818845039127691264 |
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| author | Ling Chu Jonathan Tyson Juliana E. Shaw Felix Rivera-Molina Anthony J. Koleske Alanna Schepartz Derek K. Toomre |
| author_facet | Ling Chu Jonathan Tyson Juliana E. Shaw Felix Rivera-Molina Anthony J. Koleske Alanna Schepartz Derek K. Toomre |
| author_sort | Ling Chu |
| collection | DOAJ |
| description | High density environmentally sensitive (HIDE) probes allow for long time-lapse super-resolution imaging of live cells. Here the authors develop a second HIDE probe with a bio-orthogonal labelling strategy to enable two-color nanoscopy of two organelles over extended periods. |
| first_indexed | 2024-12-19T05:23:19Z |
| format | Article |
| id | doaj.art-91511d84bdfc4dfb9363382cbb779831 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-19T05:23:19Z |
| publishDate | 2020-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-91511d84bdfc4dfb9363382cbb7798312022-12-21T20:34:27ZengNature PortfolioNature Communications2041-17232020-08-011111810.1038/s41467-020-17859-1Two-color nanoscopy of organelles for extended times with HIDE probesLing Chu0Jonathan Tyson1Juliana E. Shaw2Felix Rivera-Molina3Anthony J. Koleske4Alanna Schepartz5Derek K. Toomre6Department of Cell Biology, Yale University School of MedicineDepartment of Chemistry, Yale UniversityDepartment of Molecular Biophysics and Biochemistry, Yale UniversityDepartment of Cell Biology, Yale University School of MedicineDepartment of Molecular Biophysics and Biochemistry, Yale UniversityDepartment of Chemistry, Yale UniversityDepartment of Cell Biology, Yale University School of MedicineHigh density environmentally sensitive (HIDE) probes allow for long time-lapse super-resolution imaging of live cells. Here the authors develop a second HIDE probe with a bio-orthogonal labelling strategy to enable two-color nanoscopy of two organelles over extended periods.https://doi.org/10.1038/s41467-020-17859-1 |
| spellingShingle | Ling Chu Jonathan Tyson Juliana E. Shaw Felix Rivera-Molina Anthony J. Koleske Alanna Schepartz Derek K. Toomre Two-color nanoscopy of organelles for extended times with HIDE probes Nature Communications |
| title | Two-color nanoscopy of organelles for extended times with HIDE probes |
| title_full | Two-color nanoscopy of organelles for extended times with HIDE probes |
| title_fullStr | Two-color nanoscopy of organelles for extended times with HIDE probes |
| title_full_unstemmed | Two-color nanoscopy of organelles for extended times with HIDE probes |
| title_short | Two-color nanoscopy of organelles for extended times with HIDE probes |
| title_sort | two color nanoscopy of organelles for extended times with hide probes |
| url | https://doi.org/10.1038/s41467-020-17859-1 |
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