A Facile Sensor for Detection of Lysozyme in Egg White Based on AuNPs and Ferrocene Dicarboxylic Acid
Lysozyme (Lyz) is found in animal and human bodily fluids, and is frequently utilized as a biomarker for various diseases. Even trace amounts of Lyz in food can potentially trigger adverse immune system reactions in sensitive individuals. Therefore, it is very important to monitor Lyz concentration...
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MDPI AG
2023-03-01
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| Series: | Chemosensors |
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| Online Access: | https://www.mdpi.com/2227-9040/11/4/209 |
| _version_ | 1827745561133973504 |
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| author | Jiazheng Wan Guocheng Han |
| author_facet | Jiazheng Wan Guocheng Han |
| author_sort | Jiazheng Wan |
| collection | DOAJ |
| description | Lysozyme (Lyz) is found in animal and human bodily fluids, and is frequently utilized as a biomarker for various diseases. Even trace amounts of Lyz in food can potentially trigger adverse immune system reactions in sensitive individuals. Therefore, it is very important to monitor Lyz concentration in foods for safety. In this study, a simple and convenient electrochemical sensor for Lyz detection was prepared by modifying gold nanoparticles (AuNPs) and ferrocene dicarboxylic acid (Fc(COOH)<sub>2</sub>) on a glass carbon electrode (GCE), which was characterized fully by various electrochemical methods and field emission scanning electron microscope (FESEM). The proposed method utilized Fc(COOH)<sub>2</sub> as a probe and AuNPs as an electron transfer medium to improve the sensor’s current response performance. Under optimal conditions, the sensor was used to detect Lyz with a linear range from 0.10~0.70 mmol·L<sup>−1</sup> with a sensitivity of 50.55 μA·mM<sup>−1</sup>·cm<sup>−2</sup>, and a limit of detection (LOD) of 0.07 mmol·L<sup>−1</sup>. In the standard addition experiment of food samples (egg white), a total R.S.D. of less than 6.75% and an average recovery between 95.45% and 102.62% were obtained. |
| first_indexed | 2024-03-11T05:08:13Z |
| format | Article |
| id | doaj.art-9157d2ac43d1405abf7fb1ab23b02534 |
| institution | Directory Open Access Journal |
| issn | 2227-9040 |
| language | English |
| last_indexed | 2024-03-11T05:08:13Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
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| series | Chemosensors |
| spelling | doaj.art-9157d2ac43d1405abf7fb1ab23b025342023-11-17T18:44:26ZengMDPI AGChemosensors2227-90402023-03-0111420910.3390/chemosensors11040209A Facile Sensor for Detection of Lysozyme in Egg White Based on AuNPs and Ferrocene Dicarboxylic AcidJiazheng Wan0Guocheng Han1School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin 541004, ChinaSchool of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin 541004, ChinaLysozyme (Lyz) is found in animal and human bodily fluids, and is frequently utilized as a biomarker for various diseases. Even trace amounts of Lyz in food can potentially trigger adverse immune system reactions in sensitive individuals. Therefore, it is very important to monitor Lyz concentration in foods for safety. In this study, a simple and convenient electrochemical sensor for Lyz detection was prepared by modifying gold nanoparticles (AuNPs) and ferrocene dicarboxylic acid (Fc(COOH)<sub>2</sub>) on a glass carbon electrode (GCE), which was characterized fully by various electrochemical methods and field emission scanning electron microscope (FESEM). The proposed method utilized Fc(COOH)<sub>2</sub> as a probe and AuNPs as an electron transfer medium to improve the sensor’s current response performance. Under optimal conditions, the sensor was used to detect Lyz with a linear range from 0.10~0.70 mmol·L<sup>−1</sup> with a sensitivity of 50.55 μA·mM<sup>−1</sup>·cm<sup>−2</sup>, and a limit of detection (LOD) of 0.07 mmol·L<sup>−1</sup>. In the standard addition experiment of food samples (egg white), a total R.S.D. of less than 6.75% and an average recovery between 95.45% and 102.62% were obtained.https://www.mdpi.com/2227-9040/11/4/209electrochemical sensorsferrocene dicarboxylicgold nanoparticleslysozymeamplifier unit |
| spellingShingle | Jiazheng Wan Guocheng Han A Facile Sensor for Detection of Lysozyme in Egg White Based on AuNPs and Ferrocene Dicarboxylic Acid Chemosensors electrochemical sensors ferrocene dicarboxylic gold nanoparticles lysozyme amplifier unit |
| title | A Facile Sensor for Detection of Lysozyme in Egg White Based on AuNPs and Ferrocene Dicarboxylic Acid |
| title_full | A Facile Sensor for Detection of Lysozyme in Egg White Based on AuNPs and Ferrocene Dicarboxylic Acid |
| title_fullStr | A Facile Sensor for Detection of Lysozyme in Egg White Based on AuNPs and Ferrocene Dicarboxylic Acid |
| title_full_unstemmed | A Facile Sensor for Detection of Lysozyme in Egg White Based on AuNPs and Ferrocene Dicarboxylic Acid |
| title_short | A Facile Sensor for Detection of Lysozyme in Egg White Based on AuNPs and Ferrocene Dicarboxylic Acid |
| title_sort | facile sensor for detection of lysozyme in egg white based on aunps and ferrocene dicarboxylic acid |
| topic | electrochemical sensors ferrocene dicarboxylic gold nanoparticles lysozyme amplifier unit |
| url | https://www.mdpi.com/2227-9040/11/4/209 |
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