MVA-based vaccines are protective against lethal eastern equine encephalitis virus aerosol challenge in cynomolgus macaques
Abstract MVA-based monovalent eastern equine encephalitis virus (MVA-BN-EEEV) and multivalent western, eastern, and Venezuelan equine encephalitis virus (MVA-BN-WEV) vaccines were evaluated in the cynomolgus macaque aerosol model of EEEV infection. Macaques vaccinated with two doses of 5 × 108 infec...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-02-01
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| Series: | npj Vaccines |
| Online Access: | https://doi.org/10.1038/s41541-024-00842-y |
| _version_ | 1797276001362771968 |
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| author | Brandon J. Beddingfield Kenneth S. Plante Jessica A. Plante Scott C. Weaver Sarah Bose Clara Krzykwa Nicole Chirichella Rachel K. Redmann Stephanie Z. Seiler Jason Dufour Robert V. Blair Kathrin Endt Ariane Volkmann Nicholas J. Maness Chad J. Roy |
| author_facet | Brandon J. Beddingfield Kenneth S. Plante Jessica A. Plante Scott C. Weaver Sarah Bose Clara Krzykwa Nicole Chirichella Rachel K. Redmann Stephanie Z. Seiler Jason Dufour Robert V. Blair Kathrin Endt Ariane Volkmann Nicholas J. Maness Chad J. Roy |
| author_sort | Brandon J. Beddingfield |
| collection | DOAJ |
| description | Abstract MVA-based monovalent eastern equine encephalitis virus (MVA-BN-EEEV) and multivalent western, eastern, and Venezuelan equine encephalitis virus (MVA-BN-WEV) vaccines were evaluated in the cynomolgus macaque aerosol model of EEEV infection. Macaques vaccinated with two doses of 5 × 108 infectious units of the MVA-BN-EEEV or MVA-BN-WEV vaccine by the intramuscular route rapidly developed robust levels of neutralizing antibodies to EEEV that persisted at high levels until challenge at day 84 via small particle aerosol delivery with a target inhaled dose of 107 PFU of EEEV FL93-939. Robust protection was observed, with 7/8 animals receiving MVA-BN-EEEV and 100% (8/8) animals receiving MVA-BN-WEV surviving while only 2/8 mock vaccinated controls survived lethal challenge. Complete protection from viremia was afforded by both vaccines, with near complete protection from vRNA loads in tissues and any pathologic evidence of central nervous system damage. Overall, the results indicate both vaccines are effective in eliciting an immune response that is consistent with protection from aerosolized EEEV-induced disease. |
| first_indexed | 2024-03-07T15:22:03Z |
| format | Article |
| id | doaj.art-91786bd512c949c98f7c1d156f0d183c |
| institution | Directory Open Access Journal |
| issn | 2059-0105 |
| language | English |
| last_indexed | 2024-03-07T15:22:03Z |
| publishDate | 2024-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Vaccines |
| spelling | doaj.art-91786bd512c949c98f7c1d156f0d183c2024-03-05T17:37:16ZengNature Portfolionpj Vaccines2059-01052024-02-019111110.1038/s41541-024-00842-yMVA-based vaccines are protective against lethal eastern equine encephalitis virus aerosol challenge in cynomolgus macaquesBrandon J. Beddingfield0Kenneth S. Plante1Jessica A. Plante2Scott C. Weaver3Sarah Bose4Clara Krzykwa5Nicole Chirichella6Rachel K. Redmann7Stephanie Z. Seiler8Jason Dufour9Robert V. Blair10Kathrin Endt11Ariane Volkmann12Nicholas J. Maness13Chad J. Roy14Division of Microbiology, Tulane National Primate Research CenterDepartment of Microbiology and Immunology, University of Texas Medical BranchDepartment of Microbiology and Immunology, University of Texas Medical BranchDepartment of Microbiology and Immunology, University of Texas Medical BranchDivision of Microbiology, Tulane National Primate Research CenterDivision of Microbiology, Tulane National Primate Research CenterDivision of Microbiology, Tulane National Primate Research CenterDivision of Microbiology, Tulane National Primate Research CenterDivision of Microbiology, Tulane National Primate Research CenterDivision of Veterinary Medicine, Tulane National Primate Research CenterDivision of Comparative Pathology, Tulane National Primate Research CenterBavarian Nordic GmbHBavarian Nordic GmbHDivision of Microbiology, Tulane National Primate Research CenterDivision of Microbiology, Tulane National Primate Research CenterAbstract MVA-based monovalent eastern equine encephalitis virus (MVA-BN-EEEV) and multivalent western, eastern, and Venezuelan equine encephalitis virus (MVA-BN-WEV) vaccines were evaluated in the cynomolgus macaque aerosol model of EEEV infection. Macaques vaccinated with two doses of 5 × 108 infectious units of the MVA-BN-EEEV or MVA-BN-WEV vaccine by the intramuscular route rapidly developed robust levels of neutralizing antibodies to EEEV that persisted at high levels until challenge at day 84 via small particle aerosol delivery with a target inhaled dose of 107 PFU of EEEV FL93-939. Robust protection was observed, with 7/8 animals receiving MVA-BN-EEEV and 100% (8/8) animals receiving MVA-BN-WEV surviving while only 2/8 mock vaccinated controls survived lethal challenge. Complete protection from viremia was afforded by both vaccines, with near complete protection from vRNA loads in tissues and any pathologic evidence of central nervous system damage. Overall, the results indicate both vaccines are effective in eliciting an immune response that is consistent with protection from aerosolized EEEV-induced disease.https://doi.org/10.1038/s41541-024-00842-y |
| spellingShingle | Brandon J. Beddingfield Kenneth S. Plante Jessica A. Plante Scott C. Weaver Sarah Bose Clara Krzykwa Nicole Chirichella Rachel K. Redmann Stephanie Z. Seiler Jason Dufour Robert V. Blair Kathrin Endt Ariane Volkmann Nicholas J. Maness Chad J. Roy MVA-based vaccines are protective against lethal eastern equine encephalitis virus aerosol challenge in cynomolgus macaques npj Vaccines |
| title | MVA-based vaccines are protective against lethal eastern equine encephalitis virus aerosol challenge in cynomolgus macaques |
| title_full | MVA-based vaccines are protective against lethal eastern equine encephalitis virus aerosol challenge in cynomolgus macaques |
| title_fullStr | MVA-based vaccines are protective against lethal eastern equine encephalitis virus aerosol challenge in cynomolgus macaques |
| title_full_unstemmed | MVA-based vaccines are protective against lethal eastern equine encephalitis virus aerosol challenge in cynomolgus macaques |
| title_short | MVA-based vaccines are protective against lethal eastern equine encephalitis virus aerosol challenge in cynomolgus macaques |
| title_sort | mva based vaccines are protective against lethal eastern equine encephalitis virus aerosol challenge in cynomolgus macaques |
| url | https://doi.org/10.1038/s41541-024-00842-y |
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