MiR-221 and miR-222 target PUMA to induce cell survival in glioblastoma
<p>Abstract</p> <p>Background</p> <p>MiR-221 and miR-222 (miR-221/222) are frequently up-regulated in various types of human malignancy including glioblastoma. Recent studies have reported that miR-221/222 regulate cell growth and cell cycle progression by targeting p27...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2010-09-01
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| Series: | Molecular Cancer |
| Online Access: | http://www.molecular-cancer.com/content/9/1/229 |
| _version_ | 1819042302086086656 |
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| author | Zhang Chun-Zhi Zhang Jun-Xia Zhang An-Ling Shi Zhen-Dong Han Lei Jia Zhi-Fan Yang Wei-Dong Wang Guang-Xiu Jiang Tao You Yong-Ping Pu Pei-Yu Cheng Jin-Quan Kang Chun-Sheng |
| author_facet | Zhang Chun-Zhi Zhang Jun-Xia Zhang An-Ling Shi Zhen-Dong Han Lei Jia Zhi-Fan Yang Wei-Dong Wang Guang-Xiu Jiang Tao You Yong-Ping Pu Pei-Yu Cheng Jin-Quan Kang Chun-Sheng |
| author_sort | Zhang Chun-Zhi |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>MiR-221 and miR-222 (miR-221/222) are frequently up-regulated in various types of human malignancy including glioblastoma. Recent studies have reported that miR-221/222 regulate cell growth and cell cycle progression by targeting p27 and p57. However the underlying mechanism involved in cell survival modulation of miR-221/222 remains elusive.</p> <p>Results</p> <p>Here we showed that miR-221/222 inhibited cell apoptosis by targeting pro-apoptotic gene PUMA in human glioma cells. Enforced expression of miR-22/222 induced cell survival whereas knockdown of miR-221/222 rendered cells to apoptosis. Further, miR-221/222 reduced PUMA protein levels by targeting PUMA-3'UTR. Introducing PUMA cDNA without 3'UTR abrogated miR-221/222-induced cell survival. Notably, knockdown of miR-221/222 induces PUMA expression and cell apoptosis and considerably decreases tumor growth in xenograft model. Finally, there was an inverse relationship between PUMA and miR-221/222 expression in glioma tissues.</p> <p>Conclusion</p> <p>To our knowledge, these data indicate for the first time that miR-221/222 directly regulate apoptosis by targeting PUMA in glioblastoma and that miR-221/222 could be potential therapeutic targets for glioblastoma intervention.</p> |
| first_indexed | 2024-12-21T09:38:43Z |
| format | Article |
| id | doaj.art-9189ba54beca48af8ae69c0c04b40c80 |
| institution | Directory Open Access Journal |
| issn | 1476-4598 |
| language | English |
| last_indexed | 2024-12-21T09:38:43Z |
| publishDate | 2010-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Cancer |
| spelling | doaj.art-9189ba54beca48af8ae69c0c04b40c802022-12-21T19:08:32ZengBMCMolecular Cancer1476-45982010-09-019122910.1186/1476-4598-9-229MiR-221 and miR-222 target PUMA to induce cell survival in glioblastomaZhang Chun-ZhiZhang Jun-XiaZhang An-LingShi Zhen-DongHan LeiJia Zhi-FanYang Wei-DongWang Guang-XiuJiang TaoYou Yong-PingPu Pei-YuCheng Jin-QuanKang Chun-Sheng<p>Abstract</p> <p>Background</p> <p>MiR-221 and miR-222 (miR-221/222) are frequently up-regulated in various types of human malignancy including glioblastoma. Recent studies have reported that miR-221/222 regulate cell growth and cell cycle progression by targeting p27 and p57. However the underlying mechanism involved in cell survival modulation of miR-221/222 remains elusive.</p> <p>Results</p> <p>Here we showed that miR-221/222 inhibited cell apoptosis by targeting pro-apoptotic gene PUMA in human glioma cells. Enforced expression of miR-22/222 induced cell survival whereas knockdown of miR-221/222 rendered cells to apoptosis. Further, miR-221/222 reduced PUMA protein levels by targeting PUMA-3'UTR. Introducing PUMA cDNA without 3'UTR abrogated miR-221/222-induced cell survival. Notably, knockdown of miR-221/222 induces PUMA expression and cell apoptosis and considerably decreases tumor growth in xenograft model. Finally, there was an inverse relationship between PUMA and miR-221/222 expression in glioma tissues.</p> <p>Conclusion</p> <p>To our knowledge, these data indicate for the first time that miR-221/222 directly regulate apoptosis by targeting PUMA in glioblastoma and that miR-221/222 could be potential therapeutic targets for glioblastoma intervention.</p>http://www.molecular-cancer.com/content/9/1/229 |
| spellingShingle | Zhang Chun-Zhi Zhang Jun-Xia Zhang An-Ling Shi Zhen-Dong Han Lei Jia Zhi-Fan Yang Wei-Dong Wang Guang-Xiu Jiang Tao You Yong-Ping Pu Pei-Yu Cheng Jin-Quan Kang Chun-Sheng MiR-221 and miR-222 target PUMA to induce cell survival in glioblastoma Molecular Cancer |
| title | MiR-221 and miR-222 target PUMA to induce cell survival in glioblastoma |
| title_full | MiR-221 and miR-222 target PUMA to induce cell survival in glioblastoma |
| title_fullStr | MiR-221 and miR-222 target PUMA to induce cell survival in glioblastoma |
| title_full_unstemmed | MiR-221 and miR-222 target PUMA to induce cell survival in glioblastoma |
| title_short | MiR-221 and miR-222 target PUMA to induce cell survival in glioblastoma |
| title_sort | mir 221 and mir 222 target puma to induce cell survival in glioblastoma |
| url | http://www.molecular-cancer.com/content/9/1/229 |
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