No extraction? No problem. Direct to PCR processing of tongue swabs for diagnosis of tuberculosis disease as an alternative to sputum collection

No extraction? No problem. Direct to PCR processing of tongue swabs for diagnosis of tuberculosis disease as an alternative to sputum collection

ABSTRACT Sputum collection and testing for tuberculosis (TB) have been problematic due to the potential for aerosolization, difficulty in generating a quality sample, and complex DNA extraction methods. Tongue swabs are inexpensive, minimally invasive, and a promising alternative to sputum collectio...

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Bibliographic Details
Main Authors: Charlotte L. Ahls, Duncan Emsweller, Seth J. Helfers, Xin Niu, Douglas Wilson, Leah R. Padgett, Paul K. Drain
Format: Article
Language:English
Published: American Society for Microbiology 2024-01-01
Series:Microbiology Spectrum
Subjects:
Mycobacterium tuberculosis
tongue swab
qPCR
direct lysis
Online Access:https://journals.asm.org/doi/10.1128/spectrum.03107-23

Internet

https://journals.asm.org/doi/10.1128/spectrum.03107-23

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