Enabling multiplexed testing of pooled donor cells through whole-genome sequencing

Enabling multiplexed testing of pooled donor cells through whole-genome sequencing

Abstract We describe a method that enables the multiplex screening of a pool of many different donor cell lines. Our method accurately predicts each donor proportion from the pool without requiring the use of unique DNA barcodes as markers of donor identity. Instead, we take advantage of common sing...

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Bibliographic Details
Main Authors: Yingleong Chan, Ying Kai Chan, Daniel B. Goodman, Xiaoge Guo, Alejandro Chavez, Elaine T. Lim, George M. Church
Format: Article
Language:English
Published: BMC 2018-04-01
Series:Genome Medicine
Subjects:
Multiplexed testing
Barcode free method
Single nucleotide polymorphisms
Expectation maximization algorithm
Next-generation sequencing
Personal Genome Project
Online Access:http://link.springer.com/article/10.1186/s13073-018-0541-6

Internet

http://link.springer.com/article/10.1186/s13073-018-0541-6

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