Using Cy5-dUTP labelling of RPA-amplicons with downstream microarray analysis for the detection of antibiotic resistance genes
Abstract In this report we describe Cy5-dUTP labelling of recombinase-polymerase-amplification (RPA) products directly during the amplification process for the first time. Nucleic acid amplification techniques, especially polymerase-chain-reaction as well as various isothermal amplification methods...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
Nature Portfolio
2021-10-01
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Series: | Scientific Reports |
Online Access: | https://doi.org/10.1038/s41598-021-99774-z |