Protocol to identify the ligand binding site of Mincle using NMR spectroscopy

Protocol to identify the ligand binding site of Mincle using NMR spectroscopy

Summary: Mincle (macrophage-inducible C-type lectin, CLEC4E) is a C-type lectin immune-stimulatory receptor that can be targeted for inducing potent adjuvant effects. Mincle can recognize trehalose dimycolate and related glycolipids. Here, we present a protocol to identify the ligand binding mode of...

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Bibliographic Details
Main Authors: Atsushi Furukawa, Hiroyuki Kumeta, Takashi Saitoh, Katsumi Maenaka
Format: Article
Language:English
Published: Elsevier 2024-06-01
Series:STAR Protocols
Subjects:
Surface plasmon resonance
SPR
Protein Biochemistry
Protein expression and purification
NMR
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724001618

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http://www.sciencedirect.com/science/article/pii/S2666166724001618

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