Switch-on Fluorescence Analysis of Protease Activity with the Assistance of a Nickel Ion-Nitrilotriacetic Acid-Conjugated Magnetic Nanoparticle

Switch-on Fluorescence Analysis of Protease Activity with the Assistance of a Nickel Ion-Nitrilotriacetic Acid-Conjugated Magnetic Nanoparticle

Heterogeneous protease biosensors show high sensitivity and selectivity but usually require the immobilization of peptide substrates on a solid interface. Such methods exhibit the disadvantages of complex immobilization steps and low enzymatic efficiency induced by steric hindrance. In this work, we...

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Bibliographic Details
Main Authors: Xiaohua Ma, Yingxin Lv, Panpan Liu, Yuanqiang Hao, Ning Xia
Format: Article
Language:English
Published: MDPI AG 2023-04-01
Series:Molecules
Subjects:
proteases
caspase-3
nitrilotriacetic acid
homogeneous assays
fluorescence
magnetic nanoparticle
Online Access:https://www.mdpi.com/1420-3049/28/8/3426

Internet

https://www.mdpi.com/1420-3049/28/8/3426

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