A robust strategy for overexpression of DNA polymerase from Thermus aquaticus using an IPTG-independent autoinduction system in a benchtop bioreactor

A robust strategy for overexpression of DNA polymerase from Thermus aquaticus using an IPTG-independent autoinduction system in a benchtop bioreactor

Abstract The DNA polymerase derived from Thermus aquaticus is the most widely utilized among various DNA polymerases, indicating its significant economic importance. Consequently, efforts to achieve a substantial yield of Taq DNA polymerase (Taq-pol) are ongoing. The expression of recombinant protei...

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Bibliographic Details
Main Authors: Fina Amreta Laksmi, Kenny Lischer, Yudhi Nugraha, Wiga Alif Violando, Helbert, Isa Nuryana, Firyal Nida Khasna, Naswandi Nur, Kharisma Panji Ramadhan, Destrianti Adelina Lumban Tobing, Hariyatun, Iman Hidayat
Format: Article
Language:English
Published: Nature Portfolio 2025-02-01
Series:Scientific Reports
Subjects:
DNA polymerase
Thermus aquaticus
Autoinduction
Chemically defined medium
Escherichia coli
Online Access:https://doi.org/10.1038/s41598-025-89902-4

Internet

https://doi.org/10.1038/s41598-025-89902-4

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