Structural and Affinity Analyses of G-Quadruplex DNA Aptamers for Camptothecin Derivatives

Structural and Affinity Analyses of G-Quadruplex DNA Aptamers for Camptothecin Derivatives

We recently selected DNA aptamers that bind to camptothecin (CPT) and CPT derivatives from a 70-mer oligodeoxyribonucleotide (ODN) library using the Systematic Evolution of Ligands by EXponential enrichment (SELEX) method. The target-binding activity of the obtained 70-mer CPT-binding DNA aptamer, t...

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Bibliographic Details
Main Authors: Naoki Sugimoto, Masayasu Kuwahara, Yuuya Kasahara, Shunsuke Kitadume, Hiroaki Ozaki, Yuri Imaizumi, Hiroto Fujita
Format: Article
Language:English
Published: MDPI AG 2013-08-01
Series:Pharmaceuticals
Subjects:
DNA aptamer
G-quadruplex
camptothecin (CPT)
circular dichroism (CD) spectroscopy
fluorescence titration
fluorescence polarization (FP) assay
Online Access:http://www.mdpi.com/1424-8247/6/9/1082

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http://www.mdpi.com/1424-8247/6/9/1082

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