A rapid, simple DNA mismatch repair substrate construction method

A more flexible and higher yielding in vitro DNA mismatch repair (MMR) substrate construction method, which was developed initially by Wang and Hays, is described for the construction of a nucleotide-based chemical mismatch (G/IU) and a G/T mismatch. Our modifications use the combination of two endo...

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Bibliographic Details
Main Authors: Timothy James Kinsella, Weinan eDu
Format: Article
Language:English
Published: Frontiers Media S.A. 2011-06-01
Series:Frontiers in Oncology
Subjects:
Online Access:http://journal.frontiersin.org/Journal/10.3389/fonc.2011.00008/full