Apoptotic M540 bodies present in human semen interfere with flow cytometry-assisted assessment of sperm DNA fragmentation and oxidation

Apoptotic M540 bodies present in human semen interfere with flow cytometry-assisted assessment of sperm DNA fragmentation and oxidation

Résumé Contexte L’utilisation de la cytométrie en flux (CF) pour évaluer la fragmentation de l’ADN des spermatozoïdes via la technique TUNEL (Terminal transferase dUTP nick-end labelling) a montré des incohérences par rapport aux analyses conventionnelles par microscopie fluorescente. L’hypothèse a...

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Bibliographic Details
Main Authors: Niloofar Sadeghi, Marziyeh Tavalaee, Abbas Kiani-Esfahani, Aron Moazamian, Parviz Gharagozloo, Robert J. Aitken, Joël R. Drevet, Mohammad Hossein Nasr-Esfahani
Format: Article
Language:English
Published: BMC 2021-10-01
Series:Basic and Clinical Andrology
Subjects:
Spermatozoa
TUNEL
SCSA
8-OHdG
Merocyanine 540
Non-cellular structures
Online Access:https://doi.org/10.1186/s12610-021-00143-7

Internet

https://doi.org/10.1186/s12610-021-00143-7

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