Correlationship between self-assembly behavior and emulsion stabilization of pea protein-high methoxyl pectin complexes treated with ultrasound at pH 2.0
This study investigated the effects of ultrasound on the self-assembly behavior of pea protein (PP)-high methoxyl pectin (HMP) complexes at pH 2.0 through transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and intrinsic fluorescence analysis. The emulsion stabili...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | Ultrasonics Sonochemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1350417723003085 |
| _version_ | 1797682880427589632 |
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| author | Kaiyuan Ma Lifen Zhang Xiaoyang Sun Fusheng Chen Tingwei Zhu |
| author_facet | Kaiyuan Ma Lifen Zhang Xiaoyang Sun Fusheng Chen Tingwei Zhu |
| author_sort | Kaiyuan Ma |
| collection | DOAJ |
| description | This study investigated the effects of ultrasound on the self-assembly behavior of pea protein (PP)-high methoxyl pectin (HMP) complexes at pH 2.0 through transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and intrinsic fluorescence analysis. The emulsion stabilization mechanism of PP-HMP treated with ultrasound (PP-HMP-US) was also elucidated. The results indicated that ultrasound increased the emulsifying activity index (EAI) and emulsifying stability index (ESI) of PP-HMP. Moreover, PP-HMP-US-based emulsions formed small, dispersed oil drops, which were stable during storage. PP-HMP- and PP-HMP-US-based emulsions did not demonstrate any creaming. The TEM results revealed that ultrasound can regulate the self-assembly behavior of PP and HMP to form spherical particles with a core–shell structure. This structure possessed low turbidity, a small particle size, and high absolute zeta potential values. The FTIR and intrinsic fluorescence spectra demonstrated that ultrasound increased the α-helix and β-sheet contents and exposed the tryptophan groups to more hydrophilic environments. Ultrasound also promoted the PP-HMP self-assembly through electrostatic interaction and improved its oil–water interfacial behavior, as indicated by the EAI and ESI values of PP-HMP-US-based emulsions. The current results provide a reference for the development of an innovative emulsifier prepared by ultrasound-treated protein–pectin complexes at low pH. |
| first_indexed | 2024-03-12T00:06:20Z |
| format | Article |
| id | doaj.art-92e785666dea4162a5f65c441fbc7ad4 |
| institution | Directory Open Access Journal |
| issn | 1350-4177 |
| language | English |
| last_indexed | 2024-03-12T00:06:20Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ultrasonics Sonochemistry |
| spelling | doaj.art-92e785666dea4162a5f65c441fbc7ad42023-09-17T04:55:56ZengElsevierUltrasonics Sonochemistry1350-41772023-11-01100106596Correlationship between self-assembly behavior and emulsion stabilization of pea protein-high methoxyl pectin complexes treated with ultrasound at pH 2.0Kaiyuan Ma0Lifen Zhang1Xiaoyang Sun2Fusheng Chen3Tingwei Zhu4College of Food Science and Technology, Henan University of Technology, Zhengzhou 450001, Henan, ChinaCollege of Food Science and Technology, Henan University of Technology, Zhengzhou 450001, Henan, China; Corresponding author.College of Food and Biological Engineering, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, Henan, ChinaCollege of Food Science and Technology, Henan University of Technology, Zhengzhou 450001, Henan, ChinaCollege of Food Science and Technology, Henan University of Technology, Zhengzhou 450001, Henan, ChinaThis study investigated the effects of ultrasound on the self-assembly behavior of pea protein (PP)-high methoxyl pectin (HMP) complexes at pH 2.0 through transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and intrinsic fluorescence analysis. The emulsion stabilization mechanism of PP-HMP treated with ultrasound (PP-HMP-US) was also elucidated. The results indicated that ultrasound increased the emulsifying activity index (EAI) and emulsifying stability index (ESI) of PP-HMP. Moreover, PP-HMP-US-based emulsions formed small, dispersed oil drops, which were stable during storage. PP-HMP- and PP-HMP-US-based emulsions did not demonstrate any creaming. The TEM results revealed that ultrasound can regulate the self-assembly behavior of PP and HMP to form spherical particles with a core–shell structure. This structure possessed low turbidity, a small particle size, and high absolute zeta potential values. The FTIR and intrinsic fluorescence spectra demonstrated that ultrasound increased the α-helix and β-sheet contents and exposed the tryptophan groups to more hydrophilic environments. Ultrasound also promoted the PP-HMP self-assembly through electrostatic interaction and improved its oil–water interfacial behavior, as indicated by the EAI and ESI values of PP-HMP-US-based emulsions. The current results provide a reference for the development of an innovative emulsifier prepared by ultrasound-treated protein–pectin complexes at low pH.http://www.sciencedirect.com/science/article/pii/S1350417723003085Pea proteinHigh methoxyl pectinUltrasoundSelf-assemblyEmulsion stabilization |
| spellingShingle | Kaiyuan Ma Lifen Zhang Xiaoyang Sun Fusheng Chen Tingwei Zhu Correlationship between self-assembly behavior and emulsion stabilization of pea protein-high methoxyl pectin complexes treated with ultrasound at pH 2.0 Ultrasonics Sonochemistry Pea protein High methoxyl pectin Ultrasound Self-assembly Emulsion stabilization |
| title | Correlationship between self-assembly behavior and emulsion stabilization of pea protein-high methoxyl pectin complexes treated with ultrasound at pH 2.0 |
| title_full | Correlationship between self-assembly behavior and emulsion stabilization of pea protein-high methoxyl pectin complexes treated with ultrasound at pH 2.0 |
| title_fullStr | Correlationship between self-assembly behavior and emulsion stabilization of pea protein-high methoxyl pectin complexes treated with ultrasound at pH 2.0 |
| title_full_unstemmed | Correlationship between self-assembly behavior and emulsion stabilization of pea protein-high methoxyl pectin complexes treated with ultrasound at pH 2.0 |
| title_short | Correlationship between self-assembly behavior and emulsion stabilization of pea protein-high methoxyl pectin complexes treated with ultrasound at pH 2.0 |
| title_sort | correlationship between self assembly behavior and emulsion stabilization of pea protein high methoxyl pectin complexes treated with ultrasound at ph 2 0 |
| topic | Pea protein High methoxyl pectin Ultrasound Self-assembly Emulsion stabilization |
| url | http://www.sciencedirect.com/science/article/pii/S1350417723003085 |
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