Modular, multi‐barcode amplicon sequencing for improved species‐level detection of fungal phytopathogens: A case study of pipeline establishment targeting the Ophiostomatales

Modular, multi‐barcode amplicon sequencing for improved species‐level detection of fungal phytopathogens: A case study of pipeline establishment targeting the Ophiostomatales

Abstract Recent advances in the field of environmental DNA (eDNA) metabarcoding have produced a promising platform for early detection and broad‐spectrum monitoring of plant pests and pathogens. To date, the majority of fungal metabarcoding assays have relied upon universal primers amplifying segmen...

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Bibliographic Details
Main Authors: Conrad Trollip, Jatinder Kaur, Alexander M. Piper, Francesco Martoni, Ross Mann, Quang Dinh, Angus J. Carnegie, Brendan Rodoni, Jaqueline Edwards
Format: Article
Language:English
Published: Wiley 2024-01-01
Series:Environmental DNA
Subjects:
biosecurity
fungal diagnostics and surveillance
modular metabarcoding
secondary fungal barcode (TEF‐1a)
species‐level resolution
Online Access:https://doi.org/10.1002/edn3.368

Internet

https://doi.org/10.1002/edn3.368

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