Establishment of CRISPR/Cas9 Genome-Editing System Based on Dual sgRNAs in <i>Flammulina filiformis</i>

Establishment of CRISPR/Cas9 Genome-Editing System Based on Dual sgRNAs in <i>Flammulina filiformis</i>

<i>Flammulina filiformis</i>, previously known as Asian <i>Flammulina velutipes</i>, is one of the most commercially important edible fungi, with nutritional value and medicinal properties worldwide. However, precision genome editing using CRISPR/Cas9, which is a revolutionar...

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Bibliographic Details
Main Authors: Xiaotian Liu, Jianghan Dong, Jian Liao, Li Tian, Hao Qiu, Tao Wu, Feng Ge, Jing Zhu, Liang Shi, Ailiang Jiang, Hanshou Yu, Mingwen Zhao, Ang Ren
Format: Article
Language:English
Published: MDPI AG 2022-06-01
Series:Journal of Fungi
Subjects:
<i>Flammulina filiformis</i>
CRISPR/Cas9
gene editing
dual sgRNAs
U6 promoter
<i>pyrG</i>
Online Access:https://www.mdpi.com/2309-608X/8/7/693

Internet

https://www.mdpi.com/2309-608X/8/7/693

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