Green Synthesis of Zinc Oxide Nanoparticles Using Pomegranate Fruit Peel and Solid Coffee Grounds vs. Chemical Method of Synthesis, with Their Biocompatibility and Antibacterial Properties Investigation
This research aims to investigate the synthesis, characterization, and evaluation of the biocompatibility and antibacterial activity of novel zinc oxide (ZnO) nanoparticles (NPs) prepared by <i>Punica granatum</i> peel and <i>coffee</i> ground extracts as the reducing and cap...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/4/1236 |
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| author | Hala M. Abdelmigid Nahed Ahmed Hussien Amal Ahmed Alyamani Maissa M. Morsi Noha Moslah AlSufyani Hanan Abdulaziz kadi |
| author_facet | Hala M. Abdelmigid Nahed Ahmed Hussien Amal Ahmed Alyamani Maissa M. Morsi Noha Moslah AlSufyani Hanan Abdulaziz kadi |
| author_sort | Hala M. Abdelmigid |
| collection | DOAJ |
| description | This research aims to investigate the synthesis, characterization, and evaluation of the biocompatibility and antibacterial activity of novel zinc oxide (ZnO) nanoparticles (NPs) prepared by <i>Punica granatum</i> peel and <i>coffee</i> ground extracts as the reducing and capping agents. Chemically synthesized ZnONPs were prepared using zinc acetate dihydrate and sodium hydroxide as reducing precursors. ZnONPs were characterized using an ultraviolet-visible spectrophotometer (UV-VIS), X-ray diffraction (XRD), scanning electron microscope (SEM), transmission electron microscope (TEM), and Fourier transform infrared (FTIR) spectroscopy. Peaks of UV spectra were 300 nm for ZnONPs_PPE, 320 nm (ZnONPs_CE), 290 nm, and 440 nm (ZnONP_Chem), thereby confirming ZnONPs formation. The X-ray diffractograms revealed their hexagonal structure. TEM micrographs of the biosynthesized ZnONPs revealed their hexagonal pattern and nanorod shape for ZnONPs_Chem with particle sizes of 118.6 nm, 115.7 nm, and 111.2 nm, respectively. The FTIR analysis demonstrated the presence of proteins, carboxyl, and hydroxyl groups on ZnONPs surfaces that act as reducing and stabilizing agents. ZnONP_Chem shows the antibacterial effect on <i>Staphylococcus aureus</i>, <i>Enterobacter aerogenes</i>, <i>Pseudomonas aeruginosa</i>, and <i>Klebsiella pneumoniae</i>. <i>Punica</i> peel and coffee ground extracts are effective reducing agents for green ZnONPs synthesis with a lower cytotoxic effect on Vero cells than ZnONPs_Chem with IC50 = 111, 103, and 93 μg/mL, respectively. |
| first_indexed | 2024-03-09T21:20:58Z |
| format | Article |
| id | doaj.art-937212088f5449569d033ea110a37766 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T21:20:58Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-937212088f5449569d033ea110a377662023-11-23T21:20:54ZengMDPI AGMolecules1420-30492022-02-01274123610.3390/molecules27041236Green Synthesis of Zinc Oxide Nanoparticles Using Pomegranate Fruit Peel and Solid Coffee Grounds vs. Chemical Method of Synthesis, with Their Biocompatibility and Antibacterial Properties InvestigationHala M. Abdelmigid0Nahed Ahmed Hussien1Amal Ahmed Alyamani2Maissa M. Morsi3Noha Moslah AlSufyani4Hanan Abdulaziz kadi5Department of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Biology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Biology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Biology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaThis research aims to investigate the synthesis, characterization, and evaluation of the biocompatibility and antibacterial activity of novel zinc oxide (ZnO) nanoparticles (NPs) prepared by <i>Punica granatum</i> peel and <i>coffee</i> ground extracts as the reducing and capping agents. Chemically synthesized ZnONPs were prepared using zinc acetate dihydrate and sodium hydroxide as reducing precursors. ZnONPs were characterized using an ultraviolet-visible spectrophotometer (UV-VIS), X-ray diffraction (XRD), scanning electron microscope (SEM), transmission electron microscope (TEM), and Fourier transform infrared (FTIR) spectroscopy. Peaks of UV spectra were 300 nm for ZnONPs_PPE, 320 nm (ZnONPs_CE), 290 nm, and 440 nm (ZnONP_Chem), thereby confirming ZnONPs formation. The X-ray diffractograms revealed their hexagonal structure. TEM micrographs of the biosynthesized ZnONPs revealed their hexagonal pattern and nanorod shape for ZnONPs_Chem with particle sizes of 118.6 nm, 115.7 nm, and 111.2 nm, respectively. The FTIR analysis demonstrated the presence of proteins, carboxyl, and hydroxyl groups on ZnONPs surfaces that act as reducing and stabilizing agents. ZnONP_Chem shows the antibacterial effect on <i>Staphylococcus aureus</i>, <i>Enterobacter aerogenes</i>, <i>Pseudomonas aeruginosa</i>, and <i>Klebsiella pneumoniae</i>. <i>Punica</i> peel and coffee ground extracts are effective reducing agents for green ZnONPs synthesis with a lower cytotoxic effect on Vero cells than ZnONPs_Chem with IC50 = 111, 103, and 93 μg/mL, respectively.https://www.mdpi.com/1420-3049/27/4/1236ZnO nanoparticlesgreenchemicalVero E6MTTantibacterial activity |
| spellingShingle | Hala M. Abdelmigid Nahed Ahmed Hussien Amal Ahmed Alyamani Maissa M. Morsi Noha Moslah AlSufyani Hanan Abdulaziz kadi Green Synthesis of Zinc Oxide Nanoparticles Using Pomegranate Fruit Peel and Solid Coffee Grounds vs. Chemical Method of Synthesis, with Their Biocompatibility and Antibacterial Properties Investigation Molecules ZnO nanoparticles green chemical Vero E6 MTT antibacterial activity |
| title | Green Synthesis of Zinc Oxide Nanoparticles Using Pomegranate Fruit Peel and Solid Coffee Grounds vs. Chemical Method of Synthesis, with Their Biocompatibility and Antibacterial Properties Investigation |
| title_full | Green Synthesis of Zinc Oxide Nanoparticles Using Pomegranate Fruit Peel and Solid Coffee Grounds vs. Chemical Method of Synthesis, with Their Biocompatibility and Antibacterial Properties Investigation |
| title_fullStr | Green Synthesis of Zinc Oxide Nanoparticles Using Pomegranate Fruit Peel and Solid Coffee Grounds vs. Chemical Method of Synthesis, with Their Biocompatibility and Antibacterial Properties Investigation |
| title_full_unstemmed | Green Synthesis of Zinc Oxide Nanoparticles Using Pomegranate Fruit Peel and Solid Coffee Grounds vs. Chemical Method of Synthesis, with Their Biocompatibility and Antibacterial Properties Investigation |
| title_short | Green Synthesis of Zinc Oxide Nanoparticles Using Pomegranate Fruit Peel and Solid Coffee Grounds vs. Chemical Method of Synthesis, with Their Biocompatibility and Antibacterial Properties Investigation |
| title_sort | green synthesis of zinc oxide nanoparticles using pomegranate fruit peel and solid coffee grounds vs chemical method of synthesis with their biocompatibility and antibacterial properties investigation |
| topic | ZnO nanoparticles green chemical Vero E6 MTT antibacterial activity |
| url | https://www.mdpi.com/1420-3049/27/4/1236 |
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