Easy method for six-fragment Golden Gate Assembly of modular vectors

Efficient cloning techniques are a requirement for synthetic biology. This study provides a simplified cloning method based on Golden Gate Assembly that can be used for rapid vector construction. The building of multiple expression vectors with customizable modules is achieved in a timely manner wit...

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Bibliographic Details
Main Authors: Ionut Adrian Cepleanu-Pascu, Miruna Stan, Sebastian Cocioba, Ileana Stoica
Format: Article
Language:English
Published: Future Science Ltd 2023-02-01
Series:BioTechniques
Subjects:
Online Access:https://www.future-science.com/doi/10.2144/btn-2022-0041