Neuroprotective Effect of the Mixture of <i>Gastrodiae elata</i> and <i>Glycyrrhizae uralensis</i> In Vitro
<b>Background</b>: This study investigated the effect of a mixture of <i>Gastrodiae elata</i> and <i>Glycyrrhizae uralensis</i> (GGW) on β-amyloid-induced neuronal damage in vitro. <b>Methods</b>: For finding the appropriate GGW ratio, we performed MTT...
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MDPI AG
2022-12-01
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| Online Access: | https://www.mdpi.com/2076-3417/13/1/190 |
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| author | Su-Ha Hwang Su-Bin Park Da-Young Yu Jae-Yeon Cho Won-Woo Lee Mi-Ran Park Jang-Won Lee Yong-Deok Jeon |
| author_facet | Su-Ha Hwang Su-Bin Park Da-Young Yu Jae-Yeon Cho Won-Woo Lee Mi-Ran Park Jang-Won Lee Yong-Deok Jeon |
| author_sort | Su-Ha Hwang |
| collection | DOAJ |
| description | <b>Background</b>: This study investigated the effect of a mixture of <i>Gastrodiae elata</i> and <i>Glycyrrhizae uralensis</i> (GGW) on β-amyloid-induced neuronal damage in vitro. <b>Methods</b>: For finding the appropriate GGW ratio, we performed MTT assays using the ratios of 5:5, 6:4, 7:3, 8:2 and 9:1 in SK-N-SH cell and SH-SY-5Y cell. Treatment with β-amyloid (10 μM) caused cell death and overexpression of acetylcholinesterase (ACHE) in SH-SY-5Y cells. β-amyloid treatment increased the expression of mitogen-activated protein kinases (MAPKs). In addition, we detected the antioxidant activity of GGW using DCF-DA staining of SK-N-SH cells. To determine the effect of GGW on oxidative stress, we used a hydrogen peroxidase-induced in vitro model. <b>Results</b>: We selected the ratios of 5:5 and 7:3 mixtures with the least cytotoxicity. The 7:3 ratio of GGW (GGW73) decreased the mRNA expression of ACHE in SH-SY-5Y cell more than the 5:5 ratio of GGW (GGW55). GGW73 suppressed p-ERK protein expression in β-amyloid-treated SH-SY-5Y cells. Additionally, GGW73 regulated reactive oxygen species (ROS) in vitro. GGW73 treatment regulated apoptotic factors in β-amyloid-stimulated neuronal cells. <b>Conclusions</b>: These results suggest that GGW73 exerts neuroprotective and anti-inflammatory effects in vitro. These results also showed that GGW73 had a protective effect against H<sub>2</sub>O<sub>2</sub> induced cell damage in an in vitro model. These results indicate the potential of GGW as a therapeutic agent for cognitive disorders. |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-11T10:09:03Z |
| publishDate | 2022-12-01 |
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| series | Applied Sciences |
| spelling | doaj.art-93a852b8b5c14355a79bb0cd60761c5b2023-11-16T14:51:52ZengMDPI AGApplied Sciences2076-34172022-12-0113119010.3390/app13010190Neuroprotective Effect of the Mixture of <i>Gastrodiae elata</i> and <i>Glycyrrhizae uralensis</i> In VitroSu-Ha Hwang0Su-Bin Park1Da-Young Yu2Jae-Yeon Cho3Won-Woo Lee4Mi-Ran Park5Jang-Won Lee6Yong-Deok Jeon7Department of Korean Pharmacy, Woosuk University, 443 Samnye-ro, Samnye-eup, Wanju-Gun 55338, Jeollabuk-do, Republic of KoreaDepartment of Korean Pharmacy, Woosuk University, 443 Samnye-ro, Samnye-eup, Wanju-Gun 55338, Jeollabuk-do, Republic of KoreaDepartment of Korean Pharmacy, Woosuk University, 443 Samnye-ro, Samnye-eup, Wanju-Gun 55338, Jeollabuk-do, Republic of KoreaDepartment of Korean Pharmacy, Woosuk University, 443 Samnye-ro, Samnye-eup, Wanju-Gun 55338, Jeollabuk-do, Republic of KoreaDepartment of Korean Pharmacy, Woosuk University, 443 Samnye-ro, Samnye-eup, Wanju-Gun 55338, Jeollabuk-do, Republic of KoreaMJ Health Food Co., Ltd., 5 Sinchon-gil, Anseong-myeon, Muju-gun 55536, Jeollabuk-do, Republic of KoreaDepartment of Technical Research, Agricultural Technology Center, 416 Hanpungru-ro, Muju-eup, Muju-gun 55517, Jeollabuk-do, Republic of KoreaDepartment of Korean Pharmacy, Woosuk University, 443 Samnye-ro, Samnye-eup, Wanju-Gun 55338, Jeollabuk-do, Republic of Korea<b>Background</b>: This study investigated the effect of a mixture of <i>Gastrodiae elata</i> and <i>Glycyrrhizae uralensis</i> (GGW) on β-amyloid-induced neuronal damage in vitro. <b>Methods</b>: For finding the appropriate GGW ratio, we performed MTT assays using the ratios of 5:5, 6:4, 7:3, 8:2 and 9:1 in SK-N-SH cell and SH-SY-5Y cell. Treatment with β-amyloid (10 μM) caused cell death and overexpression of acetylcholinesterase (ACHE) in SH-SY-5Y cells. β-amyloid treatment increased the expression of mitogen-activated protein kinases (MAPKs). In addition, we detected the antioxidant activity of GGW using DCF-DA staining of SK-N-SH cells. To determine the effect of GGW on oxidative stress, we used a hydrogen peroxidase-induced in vitro model. <b>Results</b>: We selected the ratios of 5:5 and 7:3 mixtures with the least cytotoxicity. The 7:3 ratio of GGW (GGW73) decreased the mRNA expression of ACHE in SH-SY-5Y cell more than the 5:5 ratio of GGW (GGW55). GGW73 suppressed p-ERK protein expression in β-amyloid-treated SH-SY-5Y cells. Additionally, GGW73 regulated reactive oxygen species (ROS) in vitro. GGW73 treatment regulated apoptotic factors in β-amyloid-stimulated neuronal cells. <b>Conclusions</b>: These results suggest that GGW73 exerts neuroprotective and anti-inflammatory effects in vitro. These results also showed that GGW73 had a protective effect against H<sub>2</sub>O<sub>2</sub> induced cell damage in an in vitro model. These results indicate the potential of GGW as a therapeutic agent for cognitive disorders.https://www.mdpi.com/2076-3417/13/1/190<i>Gastrodiae elata</i><i>Glycyrrhizae uralensis</i>neuroprotective effectSH-SY-5Y cellanti-inflammatory effect |
| spellingShingle | Su-Ha Hwang Su-Bin Park Da-Young Yu Jae-Yeon Cho Won-Woo Lee Mi-Ran Park Jang-Won Lee Yong-Deok Jeon Neuroprotective Effect of the Mixture of <i>Gastrodiae elata</i> and <i>Glycyrrhizae uralensis</i> In Vitro Applied Sciences <i>Gastrodiae elata</i> <i>Glycyrrhizae uralensis</i> neuroprotective effect SH-SY-5Y cell anti-inflammatory effect |
| title | Neuroprotective Effect of the Mixture of <i>Gastrodiae elata</i> and <i>Glycyrrhizae uralensis</i> In Vitro |
| title_full | Neuroprotective Effect of the Mixture of <i>Gastrodiae elata</i> and <i>Glycyrrhizae uralensis</i> In Vitro |
| title_fullStr | Neuroprotective Effect of the Mixture of <i>Gastrodiae elata</i> and <i>Glycyrrhizae uralensis</i> In Vitro |
| title_full_unstemmed | Neuroprotective Effect of the Mixture of <i>Gastrodiae elata</i> and <i>Glycyrrhizae uralensis</i> In Vitro |
| title_short | Neuroprotective Effect of the Mixture of <i>Gastrodiae elata</i> and <i>Glycyrrhizae uralensis</i> In Vitro |
| title_sort | neuroprotective effect of the mixture of i gastrodiae elata i and i glycyrrhizae uralensis i in vitro |
| topic | <i>Gastrodiae elata</i> <i>Glycyrrhizae uralensis</i> neuroprotective effect SH-SY-5Y cell anti-inflammatory effect |
| url | https://www.mdpi.com/2076-3417/13/1/190 |
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