Validation of SYBR green I based closed‐tube loop‐mediated isothermal amplification (LAMP) assay for diagnosis of knowlesi malaria

Abstract Background As an alternative to PCR methods, LAMP is increasingly being used in the field of molecular diagnostics. Under isothermal conditions at 65 °C, the entire procedure takes approximately 30 min to complete. In this study, we establish a sensitive and visualized LAMP method in a clos...

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Bibliographic Details
Main Authors: Meng Yee Lai, Choo Huck Ooi, Yee Ling Lau
Format: Article
Language:English
Published: BMC 2021-03-01
Series:Malaria Journal
Subjects:
Online Access:https://doi.org/10.1186/s12936-021-03707-0