A Specific Host/Microbial Signature of Plasma-Derived Extracellular Vesicles Is Associated to Thrombosis and Marrow Fibrosis in Polycythemia Vera
Polycythemia vera is a myeloproliferative neoplasm with increased risk of thrombosis and progression to myelofibrosis. However, no disease-specific risk factors have been identified so far. Circulating extracellular vesicles (EVs) are mostly of megakaryocyte (MK-EVs) and platelet (PLT-EVs) origin an...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-10-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/13/19/4968 |
| _version_ | 1797516729152176128 |
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| author | Martina Barone Monica Barone Francesca Ricci Giuseppe Auteri Francesco Fabbri Erika Bandini Francesco Francia Pier Luigi Tazzari Nicola Vianelli Silvia Turroni Michele Cavo Lucia Catani Marco Candela Francesca Palandri |
| author_facet | Martina Barone Monica Barone Francesca Ricci Giuseppe Auteri Francesco Fabbri Erika Bandini Francesco Francia Pier Luigi Tazzari Nicola Vianelli Silvia Turroni Michele Cavo Lucia Catani Marco Candela Francesca Palandri |
| author_sort | Martina Barone |
| collection | DOAJ |
| description | Polycythemia vera is a myeloproliferative neoplasm with increased risk of thrombosis and progression to myelofibrosis. However, no disease-specific risk factors have been identified so far. Circulating extracellular vesicles (EVs) are mostly of megakaryocyte (MK-EVs) and platelet (PLT-EVs) origin and, along with phosphatidylethanolamine (PE)-EVs, play a role in cancer and thrombosis. Interestingly, circulating microbial components/microbes have been recently indicated as potential modifiers of inflammation and coagulation. Here, we investigated phenotype and microbial DNA cargo of EVs after isolation from the plasma of 38 patients with polycythemia vera. Increased proportion of MK-EVs and reduced proportion of PLT-EVs identify patients with thrombosis history. Interestingly, EVs from patients with thrombosis history were depleted in <i>Staphylococcus</i> DNA but enriched in DNA from Actinobacteria members as well as <i>Anaerococcus</i>. In addition, patients with thrombosis history had also lower levels of lipopolysaccharide-associated EVs. In regard to fibrosis, along with increased proportion of PE-EVs, the EVs of patients with marrow fibrosis were enriched in DNA from <i>Collinsella</i> and <i>Flavobacterium</i>. Here, we identified a polycythemia-vera-specific host/microbial EV-based signature associated to thrombosis history and marrow fibrosis. These data may contribute to refining PV prognosis and to identifying novel druggable targets. |
| first_indexed | 2024-03-10T07:04:56Z |
| format | Article |
| id | doaj.art-93b200e2fd21453ab449ec758d605bff |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-10T07:04:56Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-93b200e2fd21453ab449ec758d605bff2023-11-22T15:55:03ZengMDPI AGCancers2072-66942021-10-011319496810.3390/cancers13194968A Specific Host/Microbial Signature of Plasma-Derived Extracellular Vesicles Is Associated to Thrombosis and Marrow Fibrosis in Polycythemia VeraMartina Barone0Monica Barone1Francesca Ricci2Giuseppe Auteri3Francesco Fabbri4Erika Bandini5Francesco Francia6Pier Luigi Tazzari7Nicola Vianelli8Silvia Turroni9Michele Cavo10Lucia Catani11Marco Candela12Francesca Palandri13Istituto di Ematologia “Seràgnoli”, Department of Experimental, Diagnostic and Specialty Medicine, University of Bologna,40138 Bologna, ItalyDepartment of Medical and Surgical Sciences, University of Bologna, 40138 Bologna, ItalyServizio di Immunoematologia e Trasfusionale, IRCCS Azienda Ospedaliero-Universitaria di Bologna, 40138 Bologna, ItalyIstituto di Ematologia “Seràgnoli”, Department of Experimental, Diagnostic and Specialty Medicine, University of Bologna,40138 Bologna, ItalyIRCCS Istituto Romagnolo per lo Studio dei Tumori (IRST) “Dino Amadori”, 47014 Meldola, ItalyIRCCS Istituto Romagnolo per lo Studio dei Tumori (IRST) “Dino Amadori”, 47014 Meldola, ItalyDepartment of Pharmacy and Biotechnology, University of Bologna, 40138 Bologna, ItalyServizio di Immunoematologia e Trasfusionale, IRCCS Azienda Ospedaliero-Universitaria di Bologna, 40138 Bologna, ItalyIstituto di Ematologia “Seràgnoli”, IRCCS Azienda Ospedaliero-Universitaria di Bologna, 40138 Bologna, ItalyDepartment of Pharmacy and Biotechnology, University of Bologna, 40138 Bologna, ItalyIstituto di Ematologia “Seràgnoli”, Department of Experimental, Diagnostic and Specialty Medicine, University of Bologna,40138 Bologna, ItalyIstituto di Ematologia “Seràgnoli”, Department of Experimental, Diagnostic and Specialty Medicine, University of Bologna,40138 Bologna, ItalyDepartment of Pharmacy and Biotechnology, University of Bologna, 40138 Bologna, ItalyIstituto di Ematologia “Seràgnoli”, IRCCS Azienda Ospedaliero-Universitaria di Bologna, 40138 Bologna, ItalyPolycythemia vera is a myeloproliferative neoplasm with increased risk of thrombosis and progression to myelofibrosis. However, no disease-specific risk factors have been identified so far. Circulating extracellular vesicles (EVs) are mostly of megakaryocyte (MK-EVs) and platelet (PLT-EVs) origin and, along with phosphatidylethanolamine (PE)-EVs, play a role in cancer and thrombosis. Interestingly, circulating microbial components/microbes have been recently indicated as potential modifiers of inflammation and coagulation. Here, we investigated phenotype and microbial DNA cargo of EVs after isolation from the plasma of 38 patients with polycythemia vera. Increased proportion of MK-EVs and reduced proportion of PLT-EVs identify patients with thrombosis history. Interestingly, EVs from patients with thrombosis history were depleted in <i>Staphylococcus</i> DNA but enriched in DNA from Actinobacteria members as well as <i>Anaerococcus</i>. In addition, patients with thrombosis history had also lower levels of lipopolysaccharide-associated EVs. In regard to fibrosis, along with increased proportion of PE-EVs, the EVs of patients with marrow fibrosis were enriched in DNA from <i>Collinsella</i> and <i>Flavobacterium</i>. Here, we identified a polycythemia-vera-specific host/microbial EV-based signature associated to thrombosis history and marrow fibrosis. These data may contribute to refining PV prognosis and to identifying novel druggable targets.https://www.mdpi.com/2072-6694/13/19/4968polycythemia veraextracellular vesiclesmicrobial DNA cargobiomarkerthrombosismarrow fibrosis |
| spellingShingle | Martina Barone Monica Barone Francesca Ricci Giuseppe Auteri Francesco Fabbri Erika Bandini Francesco Francia Pier Luigi Tazzari Nicola Vianelli Silvia Turroni Michele Cavo Lucia Catani Marco Candela Francesca Palandri A Specific Host/Microbial Signature of Plasma-Derived Extracellular Vesicles Is Associated to Thrombosis and Marrow Fibrosis in Polycythemia Vera Cancers polycythemia vera extracellular vesicles microbial DNA cargo biomarker thrombosis marrow fibrosis |
| title | A Specific Host/Microbial Signature of Plasma-Derived Extracellular Vesicles Is Associated to Thrombosis and Marrow Fibrosis in Polycythemia Vera |
| title_full | A Specific Host/Microbial Signature of Plasma-Derived Extracellular Vesicles Is Associated to Thrombosis and Marrow Fibrosis in Polycythemia Vera |
| title_fullStr | A Specific Host/Microbial Signature of Plasma-Derived Extracellular Vesicles Is Associated to Thrombosis and Marrow Fibrosis in Polycythemia Vera |
| title_full_unstemmed | A Specific Host/Microbial Signature of Plasma-Derived Extracellular Vesicles Is Associated to Thrombosis and Marrow Fibrosis in Polycythemia Vera |
| title_short | A Specific Host/Microbial Signature of Plasma-Derived Extracellular Vesicles Is Associated to Thrombosis and Marrow Fibrosis in Polycythemia Vera |
| title_sort | specific host microbial signature of plasma derived extracellular vesicles is associated to thrombosis and marrow fibrosis in polycythemia vera |
| topic | polycythemia vera extracellular vesicles microbial DNA cargo biomarker thrombosis marrow fibrosis |
| url | https://www.mdpi.com/2072-6694/13/19/4968 |
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