An improved fluorescent noncanonical amino acid for measuring conformational distributions using time-resolved transition metal ion FRET

An improved fluorescent noncanonical amino acid for measuring conformational distributions using time-resolved transition metal ion FRET

With the recent explosion in high-resolution protein structures, one of the next frontiers in biology is elucidating the mechanisms by which conformational rearrangements in proteins are regulated to meet the needs of cells under changing conditions. Rigorously measuring protein energetics and dynam...

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Bibliographic Details
Main Authors: William N Zagotta, Brandon S Sim, Anthony K Nhim, Marium M Raza, Eric GB Evans, Yarra Venkatesh, Chloe M Jones, Ryan A Mehl, E James Petersson, Sharona E Gordon
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2021-10-01
Series:eLife
Subjects:
Fluorescence
FRET
protein dynamics
noncanonical amino acid
maltose binding protein
fluorescence lifetime
Online Access:https://elifesciences.org/articles/70236

Internet

https://elifesciences.org/articles/70236

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