High-throughput iSpinach fluorescent aptamer-based real-time monitoring of in vitro transcription

High-throughput iSpinach fluorescent aptamer-based real-time monitoring of in vitro transcription

Abstract In vitro transcription (IVT) is an essential technique for RNA synthesis. Methods for the accurate and rapid screening of IVT conditions will facilitate RNA polymerase engineering, promoter optimization, and screening for new transcription inhibitor drugs. However, traditional polyacrylamid...

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Bibliographic Details
Main Authors: Weitong Qin, Liang Li, Fan Yang, Siyuan Wang, Guang-Yu Yang
Format: Article
Language:English
Published: SpringerOpen 2022-10-01
Series:Bioresources and Bioprocessing
Subjects:
In vitro transcription
RNA aptamer
T7 RNAP
Real-time detection
Online Access:https://doi.org/10.1186/s40643-022-00598-0

Internet

https://doi.org/10.1186/s40643-022-00598-0

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