Modifications of the 5’ region of the CASPONTM tag’s mRNA further enhance soluble recombinant protein production in Escherichia coli

Modifications of the 5’ region of the CASPONTM tag’s mRNA further enhance soluble recombinant protein production in Escherichia coli

Abstract Background Escherichia coli is one of the most commonly used host organisms for the production of biopharmaceuticals, as it allows for cost-efficient and fast recombinant protein expression. However, challenging proteins are often produced with low titres or as inclusion bodies, and the man...

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Bibliographic Details
Main Authors: Christoph Köppl, Wolfgang Buchinger, Gerald Striedner, Monika Cserjan-Puschmann
Format: Article
Language:English
Published: BMC 2024-03-01
Series:Microbial Cell Factories
Subjects:
CASPONTM technology
Fusion tag
5’UTR
Recombinant protein
Rare codons
Free folding energy
Online Access:https://doi.org/10.1186/s12934-024-02350-z

Internet

https://doi.org/10.1186/s12934-024-02350-z

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