Virulence Potential of Rhizopus Species in an Embryonated Chicken Egg Model
Introduction: Little is known about the pathogenicity and virulence properties of the Rhizopus species used in food fermentation. This study aimed to investigate the virulence potential of Rhizopus arrhizus and R. microsporus strains obtained from a wide selection of clinical and environmental sourc...
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| Format: | Article |
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Ilam University of Medical Sciences
2020-06-01
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| Series: | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām |
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| Online Access: | http://sjimu.medilam.ac.ir/article-1-5686-en.html |
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| author | Somayeh Dolatabadi Zahra Pourfraidongahasrodashti Mohammad javad Najafzadeh Seyed Abolfazl Hoseyni |
| author_facet | Somayeh Dolatabadi Zahra Pourfraidongahasrodashti Mohammad javad Najafzadeh Seyed Abolfazl Hoseyni |
| author_sort | Somayeh Dolatabadi |
| collection | DOAJ |
| description | Introduction: Little is known about the pathogenicity and virulence properties of the Rhizopus species used in food fermentation. This study aimed to investigate the virulence potential of Rhizopus arrhizus and R. microsporus strains obtained from a wide selection of clinical and environmental sources in an embryonated chicken egg model.
Materials & Methods: In total, 26 strains (13 strains from each species) were inoculated in embryonated eggs with final concentration of 106 spore/ml/egg. The eggs were inoculated (0.1 ml) through chorio-allantoic membrane in day 10 and incubated at 37.6 °C and 60% humidity and monitored for 7 days. Ethics code: IR.MUMS.fm.REC.1396.457
Findings: The mortality rate for R. microsporus was slightly higher than that of R. arrhizus. The ability of R. arrhizus and R. microsporus to cause infection was strain-specific but source-independent in the embryonated chicken eggs. The presence of endosymbiont bacteria (Burkhulderia) in some strains of R. microspores did not show any effect on their virulence.
Discussion & Conclusions: The occurrence of virulence in strains isolated from fermented food indicates the opportunistic nature of these fungi and possibility of a public health risk for consumers. |
| first_indexed | 2024-04-13T06:27:41Z |
| format | Article |
| id | doaj.art-93e255e6fc8848878bf2369657e01f7f |
| institution | Directory Open Access Journal |
| issn | 1563-4728 2588-3135 |
| language | fas |
| last_indexed | 2024-04-13T06:27:41Z |
| publishDate | 2020-06-01 |
| publisher | Ilam University of Medical Sciences |
| record_format | Article |
| series | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām |
| spelling | doaj.art-93e255e6fc8848878bf2369657e01f7f2022-12-22T02:58:21ZfasIlam University of Medical SciencesMajallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām1563-47282588-31352020-06-012827282Virulence Potential of Rhizopus Species in an Embryonated Chicken Egg ModelSomayeh Dolatabadi0Zahra Pourfraidongahasrodashti1Mohammad javad Najafzadeh2Seyed Abolfazl Hoseyni3 Faculty of engineering, Sabzevar University of New Technology, Sabzevar, Iran Dept of Pathology, Faculty of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran Dept of Parasitology and Mycology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Dept of Biology, Faculty of Life Sciences, Hakim Sabzevari University, Sabzevar, Iran Introduction: Little is known about the pathogenicity and virulence properties of the Rhizopus species used in food fermentation. This study aimed to investigate the virulence potential of Rhizopus arrhizus and R. microsporus strains obtained from a wide selection of clinical and environmental sources in an embryonated chicken egg model. Materials & Methods: In total, 26 strains (13 strains from each species) were inoculated in embryonated eggs with final concentration of 106 spore/ml/egg. The eggs were inoculated (0.1 ml) through chorio-allantoic membrane in day 10 and incubated at 37.6 °C and 60% humidity and monitored for 7 days. Ethics code: IR.MUMS.fm.REC.1396.457 Findings: The mortality rate for R. microsporus was slightly higher than that of R. arrhizus. The ability of R. arrhizus and R. microsporus to cause infection was strain-specific but source-independent in the embryonated chicken eggs. The presence of endosymbiont bacteria (Burkhulderia) in some strains of R. microspores did not show any effect on their virulence. Discussion & Conclusions: The occurrence of virulence in strains isolated from fermented food indicates the opportunistic nature of these fungi and possibility of a public health risk for consumers.http://sjimu.medilam.ac.ir/article-1-5686-en.htmlfermented foodembryonated chicken egg modelmucoralesrhizopusrhizopusvirulence |
| spellingShingle | Somayeh Dolatabadi Zahra Pourfraidongahasrodashti Mohammad javad Najafzadeh Seyed Abolfazl Hoseyni Virulence Potential of Rhizopus Species in an Embryonated Chicken Egg Model Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām fermented food embryonated chicken egg model mucorales rhizopus rhizopus virulence |
| title | Virulence Potential of Rhizopus Species in an
Embryonated Chicken Egg Model |
| title_full | Virulence Potential of Rhizopus Species in an
Embryonated Chicken Egg Model |
| title_fullStr | Virulence Potential of Rhizopus Species in an
Embryonated Chicken Egg Model |
| title_full_unstemmed | Virulence Potential of Rhizopus Species in an
Embryonated Chicken Egg Model |
| title_short | Virulence Potential of Rhizopus Species in an
Embryonated Chicken Egg Model |
| title_sort | virulence potential of rhizopus species in an embryonated chicken egg model |
| topic | fermented food embryonated chicken egg model mucorales rhizopus rhizopus virulence |
| url | http://sjimu.medilam.ac.ir/article-1-5686-en.html |
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